6UMX

Structural basis for specific inhibition of extracellular activation of pro/latent myostatin by SRK-015

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	93
Detector technology	PIXEL
Collection date	2017-08-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	59.620, 110.010, 293.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.430 - 2.790
R-factor	0.2193
Rwork	0.217
R-free	0.26430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 5GGU 5f3h 3hh2 3rjr & 5NTU
RMSD bond length	0.019
RMSD bond angle	2.072
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	146.630	40.430	2.840
High resolution limit [Å]	2.790	7.560	2.790
Rmeas	0.079	0.032	1.487
Rpim	0.035	0.014	0.645
Total number of observations		12391	12976
Number of reflections	49196	2693	2467
<I/σ(I)>	14.1	40.9	1.2
Completeness [%]	100.0	100	100
Redundancy	5.1	4.6	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	296	0.1 M sodium citrate, pH 5.5, 20% PEG3350, 15% 2-propanol, 3% trimethylamine N-oxide