4EDW
Nerve Growth Factor in Complex with Fab from humanized version of mouse mAb 911 (tanezumab)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2005-12-08 |
Detector | ADSC QUANTUM 210r |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 64.700, 92.707, 252.729 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.590 - 2.480 |
R-factor | 0.2026 |
Rwork | 0.200 |
R-free | 0.24910 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.260 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.480 |
Number of reflections | 25872 |
<I/σ(I)> | 24 |
Completeness [%] | 95.2 |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.8 | 300 | PEG 5000 monomethylether, glycerol, sodium cacodylate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 300K |