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8EMC
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BU of 8emc by Molmil
CryoEM characterization of BrxL -- a unique AAA+ phage restriction Factor.
Descriptor: Protease Lon-related BREX system protein BrxL
Authors:Shen, B.W, Stoddard, B.L.
Deposit date:2022-09-27
Release date:2023-02-01
Last modified:2023-05-17
Method:ELECTRON MICROSCOPY (3.6 Å)
Cite:Structure, substrate binding and activity of a unique AAA+ protein: the BrxL phage restriction factor.
Nucleic Acids Res., 51, 2023
8EPX
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BU of 8epx by Molmil
Type IIS Restriction Endonuclease PaqCI, DNA bound
Descriptor: CALCIUM ION, DNA 1a, DNA 1b, ...
Authors:Kennedy, M.A, Stoddard, B.L.
Deposit date:2022-10-06
Release date:2023-03-22
Last modified:2023-06-07
Method:ELECTRON MICROSCOPY (3.15 Å)
Cite:Structures, activity and mechanism of the Type IIS restriction endonuclease PaqCI.
Nucleic Acids Res., 51, 2023
8EM1
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BU of 8em1 by Molmil
Type IIS Restriction Endonuclease PaqCI, DNA Unbound
Descriptor: 1,2-ETHANEDIOL, PaqCI, DNA Unbound
Authors:Kennedy, M.A, Stoddard, B.L.
Deposit date:2022-09-26
Release date:2023-03-22
Last modified:2024-04-03
Method:X-RAY DIFFRACTION (2.5 Å)
Cite:Structures, activity and mechanism of the Type IIS restriction endonuclease PaqCI.
Nucleic Acids Res., 51, 2023
8ETQ
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BU of 8etq by Molmil
Designed pentafoil knot protein folded into a trefoil knot
Descriptor: k-cTRP5
Authors:Doyle, L.A, Kibler, R.D, Bradley, P, Stoddard, B.L.
Deposit date:2022-10-17
Release date:2023-10-25
Last modified:2023-11-15
Method:X-RAY DIFFRACTION (2.42 Å)
Cite:De novo design of knotted tandem repeat proteins.
Nat Commun, 14, 2023
1QVJ
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BU of 1qvj by Molmil
structure of NUDT9 complexed with ribose-5-phosphate
Descriptor: 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, 5-O-phosphono-beta-D-ribofuranose, ADP-ribose pyrophosphatase, ...
Authors:Shen, B.W, Perraud, A.-L, Scharenberg, A.S, Stoddard, B.L.
Deposit date:2003-08-27
Release date:2003-09-16
Last modified:2023-11-15
Method:X-RAY DIFFRACTION (1.91 Å)
Cite:The crystal structure and mutational analysis of human NUDT9
J.Mol.Biol., 332, 2003
1LTQ
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BU of 1ltq by Molmil
CRYSTAL STRUCTURE OF T4 POLYNUCLEOTIDE KINASE
Descriptor: ADENOSINE-5'-DIPHOSPHATE, DIMETHYL SULFOXIDE, POLYNUCLEOTIDE KINASE
Authors:Galburt, E.A, Pelletier, J, Wilson, G, Stoddard, B.L.
Deposit date:2002-05-20
Release date:2002-10-09
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.33 Å)
Cite:Structure of a tRNA repair enzyme and molecular biology workhorse: T4 polynucleotide kinase.
Structure, 10, 2002
1M5X
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BU of 1m5x by Molmil
Crystal structure of the homing endonuclease I-MsoI bound to its DNA substrate
Descriptor: 5'-D(*CP*GP*GP*AP*AP*CP*TP*GP*TP*CP*TP*CP*AP*CP*GP*AP*CP*GP*TP*TP*CP*TP*GP*C)-3', 5'-D(*GP*CP*AP*GP*AP*AP*CP*GP*TP*CP*GP*TP*GP*AP*GP*AP*CP*AP*GP*TP*TP*CP*CP*G)-3', CALCIUM ION, ...
Authors:Chevalier, B, Turmel, M, Lemieux, C, Monnat, R.J, Stoddard, B.L.
Deposit date:2002-07-10
Release date:2003-06-03
Last modified:2024-04-03
Method:X-RAY DIFFRACTION (2.25 Å)
Cite:Flexible DNA Target Site Recognition by Divergent Homing Endonuclease Isoschizomers I-CreI and I-MsoI
J.Mol.Biol., 329, 2003
5TH3
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BU of 5th3 by Molmil
Restriction/modification system-Type II R.SwaI cleaved DNA complex
Descriptor: 1,2-ETHANEDIOL, ACETATE ION, DNA (cleaved 25-MER, ...
Authors:Shen, B.W, Stoddard, B.L.
Deposit date:2016-09-29
Release date:2016-12-21
Last modified:2024-04-03
Method:X-RAY DIFFRACTION (2.33 Å)
Cite:DNA recognition by the SwaI restriction endonuclease involves unusual distortion of an 8 base pair A:T-rich target.
Nucleic Acids Res., 45, 2017
5THG
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BU of 5thg by Molmil
Engineered variant of I-OnuI meganuclease targeting the HIV CCR5 gene; harbors 43 point mutations relative to wild-type I-OnuI
Descriptor: CALCIUM ION, DNA (29-MER), GLYCEROL, ...
Authors:Hallinan, J.P, Stoddard, B.L.
Deposit date:2016-09-29
Release date:2017-05-03
Last modified:2024-03-06
Method:X-RAY DIFFRACTION (3.106 Å)
Cite:The structural basis of altered gene specificity resulting from meganuclease and MegaTAL engineering
to be published
4YIT
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BU of 4yit by Molmil
Crystal Structure of LAGLIDADG Meganuclease I-AabMI Bound to Uncleaved DNA
Descriptor: CALCIUM ION, DNA (25-MER), DNA (25MER), ...
Authors:Hallinan, J.P, Stoddard, B.L.
Deposit date:2015-03-02
Release date:2016-03-23
Last modified:2023-09-27
Method:X-RAY DIFFRACTION (3.24 Å)
Cite:Indirect DNA Sequence Recognition and Its Impact on Nuclease Cleavage Activity.
Structure, 24, 2016
5TGQ
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BU of 5tgq by Molmil
Restriction-modification system Type II R.SwaI, DNA free
Descriptor: 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, ACETATE ION, CALCIUM ION, ...
Authors:Shen, B.W, stoddard, B.L.
Deposit date:2016-09-28
Release date:2016-12-21
Last modified:2024-03-06
Method:X-RAY DIFFRACTION (1.88 Å)
Cite:DNA recognition by the SwaI restriction endonuclease involves unusual distortion of an 8 base pair A:T-rich target.
Nucleic Acids Res., 45, 2017
1MOW
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BU of 1mow by Molmil
E-DreI
Descriptor: 5'-D(*CP*CP*AP*AP*AP*CP*TP*GP*TP*CP*TP*CP*AP*AP*GP*TP*TP*CP*CP*GP*GP*CP*G)-3', 5'-D(*CP*GP*CP*CP*GP*GP*AP*AP*CP*TP*TP*GP*AP*GP*AP*CP*AP*GP*TP*TP*TP*GP*G)-3', GLYCEROL, ...
Authors:Chevalier, B.S, Kortemme, T, Chadsey, M.S, Baker, D, Monnat Jr, R.J, Stoddard, B.L.
Deposit date:2002-09-10
Release date:2002-11-29
Last modified:2024-02-14
Method:X-RAY DIFFRACTION (2.4 Å)
Cite:Design, Activity and Structure of a Highly Specific Artificial Endonuclease
Mol.Cell, 10, 2002
5TGX
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BU of 5tgx by Molmil
Restriction/modification system-Type II R-SwaI complexed with partially cleaved DNA
Descriptor: 1,2-ETHANEDIOL, ACETATE ION, CALCIUM ION, ...
Authors:Shen, B.W, Stoddard, B.L.
Deposit date:2016-09-28
Release date:2016-12-21
Last modified:2019-12-25
Method:X-RAY DIFFRACTION (2.3 Å)
Cite:DNA recognition by the SwaI restriction endonuclease involves unusual distortion of an 8 base pair A:T-rich target.
Nucleic Acids Res., 45, 2017
1N3E
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BU of 1n3e by Molmil
Crystal structure of I-CreI bound to a palindromic DNA sequence I (palindrome of left side of wildtype DNA target sequence)
Descriptor: 5'-D(*CP*GP*AP*AP*AP*AP*CP*GP*TP*CP*GP*TP*AP*C)-3', 5'-D(P*GP*AP*CP*GP*TP*TP*TP*TP*CP*G)-3', CALCIUM ION, ...
Authors:Chevalier, B, Turmel, M, Lemieux, C, Monnat, R.J, Stoddard, B.L.
Deposit date:2002-10-28
Release date:2003-06-03
Last modified:2024-02-14
Method:X-RAY DIFFRACTION (2.5 Å)
Cite:Flexible DNA Target Site Recognition by Divergent Homing Endonuclease Isoschizomers I-CreI and I-MsoI
J.Mol.Biol., 329, 2003
4YIS
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BU of 4yis by Molmil
Crystal Structure of LAGLIDADG Meganuclease I-CpaMI Bound to Uncleaved DNA
Descriptor: 1,2-ETHANEDIOL, CALCIUM ION, DNA (28-MER), ...
Authors:Hallinan, J.P, Kaiser, B.K, Stoddard, B.L.
Deposit date:2015-03-02
Release date:2016-03-23
Last modified:2023-09-27
Method:X-RAY DIFFRACTION (2.89 Å)
Cite:Indirect DNA Sequence Recognition and Its Impact on Nuclease Cleavage Activity.
Structure, 24, 2016
1N3F
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BU of 1n3f by Molmil
Crystal structure of I-CreI bound to a palindromic DNA sequence II (palindrome of right side of wildtype DNA target sequence)
Descriptor: 5'-D(*CP*GP*AP*AP*AP*CP*TP*GP*TP*CP*TP*CP*GP*A)-3', 5'-D(P*GP*AP*CP*AP*GP*TP*TP*TP*CP*G-3'), CALCIUM ION, ...
Authors:Chevalier, B, Turmel, M, Lemieux, C, Monnat, R.J, Stoddard, B.L.
Deposit date:2002-10-28
Release date:2003-06-03
Last modified:2024-02-14
Method:X-RAY DIFFRACTION (2 Å)
Cite:Flexible DNA Target Site Recognition by Divergent Homing Endonuclease Isoschizomers I-CreI and I-MsoI
J.Mol.Biol., 329, 2003
7SQ3
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BU of 7sq3 by Molmil
Designed trefoil knot protein, variant 1
Descriptor: CHLORIDE ION, Designed trefoil knot protein, variant 1, ...
Authors:Takushi, B, Doyle, L, Stoddard, B.L, Bradley, P.
Deposit date:2021-11-04
Release date:2022-06-29
Last modified:2024-04-03
Method:X-RAY DIFFRACTION (2.453 Å)
Cite:De novo design of knotted tandem repeat proteins.
Nat Commun, 14, 2023
4YHX
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BU of 4yhx by Molmil
Crystal Structure of LAGLIDADG Meganuclease I-GpeMI Bound to Uncleaved DNA
Descriptor: CALCIUM ION, DNA (27-MER), Ribosomal protein 3/homing endonuclease-like fusion protein
Authors:Hallinan, J.P, Kaiser, B.K, Stoddard, B.L.
Deposit date:2015-02-27
Release date:2016-03-09
Last modified:2023-09-27
Method:X-RAY DIFFRACTION (2.15 Å)
Cite:Indirect DNA Sequence Recognition and Its Impact on Nuclease Cleavage Activity.
Structure, 24, 2016
4Z1Z
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BU of 4z1z by Molmil
Crystal Structure of Meganuclease I-SmaMI Bound to Uncleaveable DNA with a TTCT Central Four
Descriptor: CALCIUM ION, DNA (28-MER), GLYCEROL, ...
Authors:Hallinan, J.P, Stoddard, B.L.
Deposit date:2015-03-27
Release date:2016-03-30
Last modified:2023-09-27
Method:X-RAY DIFFRACTION (3.2 Å)
Cite:Indirect DNA Sequence Recognition and Its Impact on Nuclease Cleavage Activity.
Structure, 24, 2016
4Z20
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BU of 4z20 by Molmil
Crystal Structure of Meganuclease I-SmaMI Bound to Uncleaveable DNA with a TTGT Central Four
Descriptor: CALCIUM ION, DNA (26-MER), GLYCEROL, ...
Authors:Hallinan, J.P, Stoddard, B.L.
Deposit date:2015-03-27
Release date:2016-03-30
Last modified:2023-09-27
Method:X-RAY DIFFRACTION (3.2 Å)
Cite:Indirect DNA Sequence Recognition and Its Impact on Nuclease Cleavage Activity.
Structure, 24, 2016
4YXX
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BU of 4yxx by Molmil
Computationally designed left-handed alpha/alpha toroid with 6 repeats
Descriptor: dTor_6x35L
Authors:Doyle, L, Bolduc, J, Stoddard, B.L, Bradley, P.
Deposit date:2015-03-23
Release date:2015-12-16
Last modified:2024-02-28
Method:X-RAY DIFFRACTION (2.18 Å)
Cite:Rational design of alpha-helical tandem repeat proteins with closed architectures.
Nature, 528, 2015
4YXY
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BU of 4yxy by Molmil
Computationally designed left-handed alpha/alpha toroid with 9 repeats; two linked rings of 12 repeats each structure
Descriptor: dTor_9x31L
Authors:Doyle, L, Stoddard, B.L, Bradley, P.
Deposit date:2015-03-23
Release date:2015-12-16
Last modified:2024-03-06
Method:X-RAY DIFFRACTION (3.203 Å)
Cite:Rational design of alpha-helical tandem repeat proteins with closed architectures.
Nature, 528, 2015
4YY5
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BU of 4yy5 by Molmil
Computationally designed left-handed alpha/alpha toroid with 3 repeats in space group P43212
Descriptor: dTor_3x33L
Authors:Hallinan, J.P, Bradley, P, Stoddard, B.L.
Deposit date:2015-03-23
Release date:2015-12-16
Last modified:2024-03-06
Method:X-RAY DIFFRACTION (2.78 Å)
Cite:Rational design of alpha-helical tandem repeat proteins with closed architectures.
Nature, 528, 2015
4YXZ
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BU of 4yxz by Molmil
Computationally designed left-handed alpha/alpha toroid with 9 repeats
Descriptor: dTor_9x31L
Authors:Doyle, L, Stoddard, B.L, Bradley, P.
Deposit date:2015-03-23
Release date:2015-12-16
Last modified:2024-03-06
Method:X-RAY DIFFRACTION (2.496 Å)
Cite:Rational design of alpha-helical tandem repeat proteins with closed architectures.
Nature, 528, 2015
4YY2
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BU of 4yy2 by Molmil
Computationally designed left-handed alpha/alpha toroid with 3 repeats in space group P212121
Descriptor: SODIUM ION, dTor_3x33L
Authors:Hallinan, J.P, Bradley, P, Stoddard, B.L.
Deposit date:2015-03-23
Release date:2015-12-16
Last modified:2024-03-06
Method:X-RAY DIFFRACTION (1.854 Å)
Cite:Rational design of alpha-helical tandem repeat proteins with closed architectures.
Nature, 528, 2015

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