4Z20

Crystal Structure of Meganuclease I-SmaMI Bound to Uncleaveable DNA with a TTGT Central Four

Summary for 4Z20

• Entry DOI: 10.2210/pdb4z20/pdb
• Related: 4YHX 4YIS 4YIT 4Z1Z 5ESP
• Descriptor: MEGANUCLEASE I-SMAMI, DNA (26-MER), CALCIUM ION, ... (6 entities in total)
• Functional Keywords: hydrolase-dna complex, laglidadg, homing endonuclease, meganuclease, hydrolase/dna
• Biological source: Sordaria macrospora (strain ATCC MYA-333 / DSM 997 / K(L3346) / K-hell); More
• Total number of polymer chains: 6
• Total formula weight: 100876.73

Authors

Hallinan, J.P.,Stoddard, B.L. (deposition date: 2015-03-27, release date: 2016-03-30, Last modification date: 2023-09-27)

• Primary citation: Lambert, A.R.,Hallinan, J.P.,Shen, B.W.,Chik, J.K.,Bolduc, J.M.,Kulshina, N.,Robins, L.I.,Kaiser, B.K.,Jarjour, J.,Havens, K.,Scharenberg, A.M.,Stoddard, B.L.; Indirect DNA Sequence Recognition and Its Impact on Nuclease Cleavage Activity.; Structure, 24:862-873, 2016; PubMed: 27133026; DOI: 10.1016/j.str.2016.03.024

Experimental method

X-RAY DIFFRACTION (3.2 Å)