4Z20
Crystal Structure of Meganuclease I-SmaMI Bound to Uncleaveable DNA with a TTGT Central Four
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D (A, D) | MEGANUCLEASE I-SMAMI | polymer | 303 | 34283.8 | 2 | UniProt (F7WD42) Pfam (PF00961) | Sordaria macrospora (strain ATCC MYA-333 / DSM 997 / K(L3346) / K-hell) | |
| 2 | B, E (C, F) | DNA (26-MER) | polymer | 26 | 7850.1 | 2 | synthetic construct | ||
| 3 | C, F (B, E) | DNA (26-MER) | polymer | 26 | 8126.3 | 2 | synthetic construct | ||
| 4 | G, J (A, D) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 5 | H, I, K (C, D, F) | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
| 6 | L, M, N, O, P (A, C, B, D, F) | water | water | 18.0 | 11 | Chemie (HOH) |
Sequence modifications
A, D: 1 - 302 (UniProt: F7WD42)
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | initiating methionine |
| Asn 165 | Leu 278 | conflict |
| Gln 267 | Met 380 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 100520.3 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 356.4 | |
| All* | Total formula weight | 100876.7 |
