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5GW7
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BU of 5gw7 by Molmil
Crystal structure of the glycosynthase mutant E727A of Escherichia coli GH63 glycosidase in complex with glucose and lactose
Descriptor: CALCIUM ION, Glucosidase YgjK, MAGNESIUM ION, ...
Authors:Miyazaki, T, Tonozuka, T.
Deposit date:2016-09-08
Release date:2016-10-12
Last modified:2023-11-08
Method:X-RAY DIFFRACTION (2.2 Å)
Cite:Crystal structure of the enzyme-product complex reveals sugar ring distortion during catalysis by family 63 inverting alpha-glycosidase
J.Struct.Biol., 196, 2016
4WVC
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BU of 4wvc by Molmil
Crystal structure of GH63 mannosylglycerate hydrolase from Thermus thermophilus HB8 in complex with Tris and D-glycerate
Descriptor: (2R)-2,3-DIHYDROXYPROPANOIC ACID, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, CHLORIDE ION, ...
Authors:Miyazaki, T, Ichikawa, M, Nishikawa, A, Tonozuka, T.
Deposit date:2014-11-05
Release date:2015-03-11
Last modified:2024-03-20
Method:X-RAY DIFFRACTION (2.1 Å)
Cite:Crystal structure and substrate-binding mode of GH63 mannosylglycerate hydrolase from Thermus thermophilus HB8.
J.Struct.Biol., 190, 2015
4WVA
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BU of 4wva by Molmil
Crystal structure of GH63 mannosylglycerate hydrolase from Thermus thermophilus HB8 in complex with Tris
Descriptor: (4S)-2-METHYL-2,4-PENTANEDIOL, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, CHLORIDE ION, ...
Authors:Miyazaki, T, Ichikawa, M, Nishikawa, A, Tonozuka, T.
Deposit date:2014-11-05
Release date:2015-03-11
Last modified:2023-11-08
Method:X-RAY DIFFRACTION (1.67 Å)
Cite:Crystal structure and substrate-binding mode of GH63 mannosylglycerate hydrolase from Thermus thermophilus HB8.
J.Struct.Biol., 190, 2015
4WVB
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BU of 4wvb by Molmil
Crystal structure of GH63 mannosylglycerate hydrolase from Thermus thermophilus HB8 in complex with glucose
Descriptor: (4S)-2-METHYL-2,4-PENTANEDIOL, CHLORIDE ION, Uncharacterized protein, ...
Authors:Miyazaki, T, Ichikawa, M, Nishikawa, A, Tonozuka, T.
Deposit date:2014-11-05
Release date:2015-03-11
Last modified:2023-11-08
Method:X-RAY DIFFRACTION (1.77 Å)
Cite:Crystal structure and substrate-binding mode of GH63 mannosylglycerate hydrolase from Thermus thermophilus HB8.
J.Struct.Biol., 190, 2015
4XPO
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BU of 4xpo by Molmil
Crystal structure of a novel alpha-galactosidase from Pedobacter saltans
Descriptor: 1,2-ETHANEDIOL, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, Alpha-glucosidase
Authors:Miyazaki, T, Ishizaki, Y, Ichikawa, M, Nishikawa, A, Tonozuka, T.
Deposit date:2015-01-17
Release date:2015-05-20
Last modified:2024-03-20
Method:X-RAY DIFFRACTION (2.1 Å)
Cite:Structural and biochemical characterization of novel bacterial alpha-galactosidases belonging to glycoside hydrolase family 31
Biochem.J., 469, 2015
4XPR
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BU of 4xpr by Molmil
Crystal structure of the mutant D365A of Pedobacter saltans GH31 alpha-galactosidase
Descriptor: 1,2-ETHANEDIOL, Alpha-glucosidase
Authors:Miyazaki, T, Ishizaki, Y, Ichikawa, M, Nishikawa, A, Tonozuka, T.
Deposit date:2015-01-17
Release date:2015-05-20
Last modified:2023-11-08
Method:X-RAY DIFFRACTION (2.01 Å)
Cite:Structural and biochemical characterization of novel bacterial alpha-galactosidases belonging to glycoside hydrolase family 31
Biochem.J., 469, 2015
4XPP
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BU of 4xpp by Molmil
Crystal structure of Pedobacter saltans GH31 alpha-galactosidase complexed with D-galactose
Descriptor: 1,2-ETHANEDIOL, Alpha-glucosidase, beta-D-galactopyranose
Authors:Miyazaki, T, Ishizaki, Y, Ichikawa, M, Nishikawa, A, Tonozuka, T.
Deposit date:2015-01-17
Release date:2015-05-20
Last modified:2023-11-08
Method:X-RAY DIFFRACTION (2.3 Å)
Cite:Structural and biochemical characterization of novel bacterial alpha-galactosidases belonging to glycoside hydrolase family 31
Biochem.J., 469, 2015
4XPQ
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BU of 4xpq by Molmil
Crystal structure of Pedobacter saltans GH31 alpha-galactosidase complexed with L-fucose
Descriptor: 1,2-ETHANEDIOL, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, Alpha-glucosidase, ...
Authors:Miyazaki, T, Ishizaki, Y, Ichikawa, M, Nishikawa, A, Tonozuka, T.
Deposit date:2015-01-17
Release date:2015-05-20
Last modified:2023-11-08
Method:X-RAY DIFFRACTION (1.85 Å)
Cite:Structural and biochemical characterization of novel bacterial alpha-galactosidases belonging to glycoside hydrolase family 31
Biochem.J., 469, 2015
4XPS
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BU of 4xps by Molmil
Crystal structure of the mutant D365A of Pedobacter saltans GH31 alpha-galactosidase complexed with p-nitrophenyl-alpha-galactopyranoside
Descriptor: 1,2-ETHANEDIOL, Alpha-glucosidase, P-NITROPHENOL, ...
Authors:Miyazaki, T, Ishizaki, Y, Ichikawa, M, Nishikawa, A, Tonozuka, T.
Deposit date:2015-01-17
Release date:2015-05-20
Last modified:2023-11-08
Method:X-RAY DIFFRACTION (2.1 Å)
Cite:Structural and biochemical characterization of novel bacterial alpha-galactosidases belonging to glycoside hydrolase family 31
Biochem.J., 469, 2015
5CA3
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BU of 5ca3 by Molmil
Crystal structure of the glycosynthase mutant D324N of Escherichia coli GH63 glycosidase in complex with glucose and lactose
Descriptor: CALCIUM ION, Glucosidase YgjK, MAGNESIUM ION, ...
Authors:Miyazaki, T, Tonozuka, T.
Deposit date:2015-06-29
Release date:2016-08-10
Last modified:2023-11-08
Method:X-RAY DIFFRACTION (1.8 Å)
Cite:Crystal structure of the enzyme-product complex reveals sugar ring distortion during catalysis by family 63 inverting alpha-glycosidase.
J.Struct.Biol., 2016
6LGD
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BU of 6lgd by Molmil
Bombyx mori GH13 sucrose hydrolase complexed with 1,4-dideoxy-1,4-imino-D-arabinitol
Descriptor: 1,4-DIDEOXY-1,4-IMINO-D-ARABINITOL, CALCIUM ION, GLYCEROL, ...
Authors:Miyazaki, T.
Deposit date:2019-12-05
Release date:2020-05-20
Last modified:2023-11-22
Method:X-RAY DIFFRACTION (1.75 Å)
Cite:Structure-function analysis of silkworm sucrose hydrolase uncovers the mechanism of substrate specificity in GH13 subfamily 17exo-alpha-glucosidases.
J.Biol.Chem., 295, 2020
6LGA
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BU of 6lga by Molmil
Bombyx mori GH13 sucrose hydrolase
Descriptor: CALCIUM ION, GLYCEROL, MAGNESIUM ION, ...
Authors:Miyazaki, T.
Deposit date:2019-12-05
Release date:2020-05-20
Last modified:2023-11-22
Method:X-RAY DIFFRACTION (1.85 Å)
Cite:Structure-function analysis of silkworm sucrose hydrolase uncovers the mechanism of substrate specificity in GH13 subfamily 17exo-alpha-glucosidases.
J.Biol.Chem., 295, 2020
6LGH
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BU of 6lgh by Molmil
Bombyx mori GH13 sucrose hydrolase mutant E322Q covalent intermediate
Descriptor: CALCIUM ION, MAGNESIUM ION, Sucrose hydrolase, ...
Authors:Miyazaki, T.
Deposit date:2019-12-05
Release date:2020-05-20
Last modified:2023-11-22
Method:X-RAY DIFFRACTION (1.7 Å)
Cite:Structure-function analysis of silkworm sucrose hydrolase uncovers the mechanism of substrate specificity in GH13 subfamily 17exo-alpha-glucosidases.
J.Biol.Chem., 295, 2020
6LGG
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BU of 6lgg by Molmil
Bombyx mori GH13 sucrose hydrolase mutant E322Q complexed with sucrose
Descriptor: CALCIUM ION, MAGNESIUM ION, Sucrose hydrolase, ...
Authors:Miyazaki, T.
Deposit date:2019-12-05
Release date:2020-05-20
Last modified:2023-11-22
Method:X-RAY DIFFRACTION (1.84 Å)
Cite:Structure-function analysis of silkworm sucrose hydrolase uncovers the mechanism of substrate specificity in GH13 subfamily 17exo-alpha-glucosidases.
J.Biol.Chem., 295, 2020
6LGI
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BU of 6lgi by Molmil
Bombyx mori GH13 sucrose hydrolase mutant E322Q covalent intermediate complexed with fructose
Descriptor: ACETATE ION, CALCIUM ION, MAGNESIUM ION, ...
Authors:Miyazaki, T.
Deposit date:2019-12-05
Release date:2020-05-20
Last modified:2023-11-22
Method:X-RAY DIFFRACTION (1.6 Å)
Cite:Structure-function analysis of silkworm sucrose hydrolase uncovers the mechanism of substrate specificity in GH13 subfamily 17exo-alpha-glucosidases.
J.Biol.Chem., 295, 2020
6LGC
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BU of 6lgc by Molmil
Bombyx mori GH13 sucrose hydrolase complexed with 1-deoxynojirimycin
Descriptor: 1-DEOXYNOJIRIMYCIN, CALCIUM ION, GLYCEROL, ...
Authors:Miyazaki, T.
Deposit date:2019-12-05
Release date:2020-05-20
Last modified:2023-11-22
Method:X-RAY DIFFRACTION (1.9 Å)
Cite:Structure-function analysis of silkworm sucrose hydrolase uncovers the mechanism of substrate specificity in GH13 subfamily 17exo-alpha-glucosidases.
J.Biol.Chem., 295, 2020
6LGE
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BU of 6lge by Molmil
Bombyx mori GH13 sucrose hydrolase complexed with acarbose
Descriptor: 4,6-dideoxy-4-{[(1S,4R,5S,6S)-4,5,6-trihydroxy-3-(hydroxymethyl)cyclohex-2-en-1-yl]amino}-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-beta-D-glucopyranose, CALCIUM ION, MAGNESIUM ION, ...
Authors:Miyazaki, T.
Deposit date:2019-12-05
Release date:2020-05-20
Last modified:2023-11-22
Method:X-RAY DIFFRACTION (1.75 Å)
Cite:Structure-function analysis of silkworm sucrose hydrolase uncovers the mechanism of substrate specificity in GH13 subfamily 17exo-alpha-glucosidases.
J.Biol.Chem., 295, 2020
6LGF
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BU of 6lgf by Molmil
Bombyx mori GH13 sucrose hydrolase mutant D247N complexed with sucrose
Descriptor: CALCIUM ION, MAGNESIUM ION, Sucrose hydrolase, ...
Authors:Miyazaki, T.
Deposit date:2019-12-05
Release date:2020-05-20
Last modified:2023-11-22
Method:X-RAY DIFFRACTION (1.85 Å)
Cite:Structure-function analysis of silkworm sucrose hydrolase uncovers the mechanism of substrate specificity in GH13 subfamily 17exo-alpha-glucosidases.
J.Biol.Chem., 295, 2020
6LGB
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BU of 6lgb by Molmil
Bombyx mori GH13 sucrose hydrolase complexed with glucose
Descriptor: CALCIUM ION, GLYCEROL, MAGNESIUM ION, ...
Authors:Miyazaki, T.
Deposit date:2019-12-05
Release date:2020-05-20
Last modified:2023-11-22
Method:X-RAY DIFFRACTION (1.7 Å)
Cite:Structure-function analysis of silkworm sucrose hydrolase uncovers the mechanism of substrate specificity in GH13 subfamily 17exo-alpha-glucosidases.
J.Biol.Chem., 295, 2020
6M76
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BU of 6m76 by Molmil
GH31 alpha-N-acetylgalactosaminidase from Enterococcus faecalis
Descriptor: 1,2-ETHANEDIOL, LPXTG-motif cell wall anchor domain protein
Authors:Miyazaki, T.
Deposit date:2020-03-17
Release date:2020-05-20
Last modified:2024-03-27
Method:X-RAY DIFFRACTION (1.4 Å)
Cite:Crystal structure of the Enterococcus faecalis alpha-N-acetylgalactosaminidase, a member of the glycoside hydrolase family 31.
Febs Lett., 594, 2020
6M77
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BU of 6m77 by Molmil
GH31 alpha-N-acetylgalactosaminidase from Enterococcus faecalis in complex with N-acetylgalactosamine
Descriptor: 1,2-ETHANEDIOL, 2-acetamido-2-deoxy-beta-D-galactopyranose, CALCIUM ION, ...
Authors:Miyazaki, T.
Deposit date:2020-03-17
Release date:2020-05-20
Last modified:2023-11-29
Method:X-RAY DIFFRACTION (1.9 Å)
Cite:Crystal structure of the Enterococcus faecalis alpha-N-acetylgalactosaminidase, a member of the glycoside hydrolase family 31.
Febs Lett., 594, 2020
3A0J
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BU of 3a0j by Molmil
Crystal structure of cold shock protein 1 from Thermus thermophilus HB8
Descriptor: Cold shock protein
Authors:Miyazaki, T, Nakagawa, N, Kuramitsu, S, Masui, R, Yokoyama, S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)
Deposit date:2009-03-19
Release date:2010-03-23
Last modified:2023-11-01
Method:X-RAY DIFFRACTION (1.65 Å)
Cite:The Biological Action of Cold Shock Protein 1 from Thermus thermophilus HB8
To be Published
7BWB
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BU of 7bwb by Molmil
Bombyx mori GH32 beta-fructofuranosidase BmSUC1
Descriptor: Beta-fructofuranosidase
Authors:Miyazaki, T, Oba, N.
Deposit date:2020-04-14
Release date:2020-11-04
Last modified:2023-11-29
Method:X-RAY DIFFRACTION (1.8 Å)
Cite:Structural insight into the substrate specificity of Bombyx mori beta-fructofuranosidase belonging to the glycoside hydrolase family 32.
Insect Biochem.Mol.Biol., 127, 2020
7BWC
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BU of 7bwc by Molmil
Bombyx mori GH32 beta-fructofuranosidase BmSUC1 mutant D63A in complex with sucrose
Descriptor: Beta-fructofuranosidase, beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose
Authors:Miyazaki, T, Oba, N.
Deposit date:2020-04-14
Release date:2020-11-04
Last modified:2023-11-29
Method:X-RAY DIFFRACTION (1.95 Å)
Cite:Structural insight into the substrate specificity of Bombyx mori beta-fructofuranosidase belonging to the glycoside hydrolase family 32.
Insect Biochem.Mol.Biol., 127, 2020
7F7Q
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BU of 7f7q by Molmil
Enterococcus faecalis GH31 alpha-N-acetylgalactosaminidase D455A in complex with p-nitrophenyl alpha-N-acetylgalactosaminide
Descriptor: 1,2-ETHANEDIOL, 2-acetamido-2-deoxy-alpha-D-galactopyranose, GH31 alpha-N-acetylgalactosaminidase, ...
Authors:Miyazaki, T.
Deposit date:2021-06-30
Release date:2021-12-08
Last modified:2023-11-29
Method:X-RAY DIFFRACTION (1.42 Å)
Cite:Structural and mechanistic insights into the substrate specificity and hydrolysis of GH31 alpha-N-acetylgalactosaminidase.
Biochimie, 195, 2022

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