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Holo structure of engineered TrpB, 2B9-H275E, from Pyrococcus furiosus in the extended-open conformation
Descriptor:	PHOSPHATE ION, SODIUM ION, Tryptophan synthase beta chain 1
Authors:	Higgins, P.M, Buller, A.R.
Deposit date:	2021-07-29
Release date:	2022-08-03
Last modified:	2023-11-15
Method:	X-RAY DIFFRACTION (2.102 Å)
Cite:	Substrate multiplexed protein engineering facilitates promiscuous biocatalytic synthesis. Nat Commun, 13, 2022

Engineered tryptophan synthase b-subunit from Pyrococcus furiosus, PfTrpB2B9-H275E with L-Trp non-covalently bound
Descriptor:	SODIUM ION, TRYPTOPHAN, Tryptophan synthase beta chain 1
Authors:	Higgins, P.M, Buller, A.R.
Deposit date:	2021-07-30
Release date:	2022-08-03
Last modified:	2023-11-15
Method:	X-RAY DIFFRACTION (2.39 Å)
Cite:	Substrate multiplexed protein engineering facilitates promiscuous biocatalytic synthesis. Nat Commun, 13, 2022

Engineered tryptophan synthase b-subunit from Pyrococcus furiosus, PfTrpB2B9_H275E with 4-Cl-Trp non-covalently bound
Descriptor:	4-chloro-L-tryptophan, SODIUM ION, Tryptophan synthase beta chain 1
Authors:	Higgins, P.M, Buller, A.R.
Deposit date:	2021-07-29
Release date:	2022-08-03
Last modified:	2023-11-15
Method:	X-RAY DIFFRACTION (2.25 Å)
Cite:	Substrate multiplexed protein engineering facilitates promiscuous biocatalytic synthesis. Nat Commun, 13, 2022