6H0A
| Serum paraoxonase-1 by directed evolution with the L69G/H115W/H134R/F222S/T332S mutations | 分子名称: | 2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, BROMIDE ION, CALCIUM ION, ... | 著者 | Ben-David, M, Sussman, J.L, Tawfik, D.S. | 登録日 | 2018-07-07 | 公開日 | 2019-07-17 | 最終更新日 | 2020-04-08 | 実験手法 | X-RAY DIFFRACTION (2.1 Å) | 主引用文献 | Enzyme Evolution: An Epistatic Ratchet versus a Smooth Reversible Transition. Mol.Biol.Evol., 37, 2020
|
|
6G82
| |
6GBK
| Repertoires of functionally diverse enzymes through computational design at epistatic active-site positions | 分子名称: | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, FORMIC ACID, Parathion hydrolase, ... | 著者 | Khersonsky, O, Lipsh, R, Avizemer, Z, Goldsmith, M, Ashani, Y, Leader, H, Dym, O, Rogotner, S, Trudeau, D, Tawfik, D.S, Fleishman, S.J. | 登録日 | 2018-04-15 | 公開日 | 2018-10-24 | 最終更新日 | 2024-01-17 | 実験手法 | X-RAY DIFFRACTION (1.9 Å) | 主引用文献 | Automated Design of Efficient and Functionally Diverse Enzyme Repertoires. Mol. Cell, 72, 2018
|
|
6GBL
| Repertoires of functionally diverse enzymes through computational design at epistatic active-site positions | 分子名称: | 1,2-ETHANEDIOL, CACODYLATE ION, FORMIC ACID, ... | 著者 | Khersonsky, O, Lipsh, R, Avizemer, Z, Goldsmith, M, Ashani, Y, Leader, H, Dym, O, Rogotner, S, Trudeau, D, Tawfik, D.S, Fleishman, S.J. | 登録日 | 2018-04-15 | 公開日 | 2018-10-24 | 最終更新日 | 2024-01-17 | 実験手法 | X-RAY DIFFRACTION (1.95 Å) | 主引用文献 | Automated Design of Efficient and Functionally Diverse Enzyme Repertoires. Mol. Cell, 72, 2018
|
|
6GVS
| Engineered glycolyl-CoA reductase comprising 8 mutations with bound NADP+ | 分子名称: | Aldehyde dehydrogenase, NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, POTASSIUM ION | 著者 | Zarzycki, J, Trudeau, D, Scheffen, M, Erb, T.J, Tawfik, D.S. | 登録日 | 2018-06-21 | 公開日 | 2018-11-28 | 最終更新日 | 2024-01-17 | 実験手法 | X-RAY DIFFRACTION (2.579 Å) | 主引用文献 | Design and in vitro realization of carbon-conserving photorespiration. Proc. Natl. Acad. Sci. U.S.A., 115, 2018
|
|
5WL8
| Crystal structure of chalcone isomerase engineered from ancestral inference (epR4) | 分子名称: | Engineered Chalcone Isomerase epR4, FORMIC ACID | 著者 | Burke, J.R, Kaltenbach, M, Tawfik, D.S, Noel, J.P. | 登録日 | 2017-07-25 | 公開日 | 2018-05-09 | 最終更新日 | 2023-10-04 | 実験手法 | X-RAY DIFFRACTION (1.58 Å) | 主引用文献 | Evolution of chalcone isomerase from a noncatalytic ancestor. Nat. Chem. Biol., 14, 2018
|
|
5WKR
| Crystal structure of chalcone isomerase engineered from ancestral inference complexed with naringenin (ancCC) | 分子名称: | (4S)-2-METHYL-2,4-PENTANEDIOL, CHLORIDE ION, Engineered Chalcone Isomerase ancCC, ... | 著者 | Burke, J.R, Kaltenbach, M, Tawfik, D.S, Noel, J.P. | 登録日 | 2017-07-25 | 公開日 | 2018-05-09 | 最終更新日 | 2023-10-04 | 実験手法 | X-RAY DIFFRACTION (1.4 Å) | 主引用文献 | Evolution of chalcone isomerase from a noncatalytic ancestor. Nat. Chem. Biol., 14, 2018
|
|
5WL4
| Crystal structure of chalcone isomerase engineered from ancestral inference (ancR3) | 分子名称: | Engineered Chalcone Isomerase ancR3, FORMIC ACID | 著者 | Burke, J.R, Kaltenbach, M, Tawfik, D.S, Noel, J.P. | 登録日 | 2017-07-25 | 公開日 | 2018-05-09 | 最終更新日 | 2023-10-04 | 実験手法 | X-RAY DIFFRACTION (2 Å) | 主引用文献 | Evolution of chalcone isomerase from a noncatalytic ancestor. Nat. Chem. Biol., 14, 2018
|
|
5WL6
| Crystal structure of chalcone isomerase engineered from ancestral inference (AncR7) | 分子名称: | CHLORIDE ION, Engineered Chalcone Isomerase AncR7 | 著者 | Burke, J.R, Kaltenbach, M, Tawfik, D.S, Noel, J.P. | 登録日 | 2017-07-25 | 公開日 | 2018-05-09 | 最終更新日 | 2023-10-04 | 実験手法 | X-RAY DIFFRACTION (1.6 Å) | 主引用文献 | Evolution of chalcone isomerase from a noncatalytic ancestor. Nat. Chem. Biol., 14, 2018
|
|
5WL3
| Crystal structure of chalcone isomerase engineered from ancestral inference (ancR2) | 分子名称: | CHLORIDE ION, Engineered Chalcone Isomerase ancR2 | 著者 | Burke, J.R, Kaltenbach, M, Tawfik, D.S, Noel, J.P. | 登録日 | 2017-07-25 | 公開日 | 2018-05-09 | 最終更新日 | 2023-10-04 | 実験手法 | X-RAY DIFFRACTION (2.4 Å) | 主引用文献 | Evolution of chalcone isomerase from a noncatalytic ancestor. Nat. Chem. Biol., 14, 2018
|
|
5WKS
| Crystal structure of chalcone isomerase engineered from ancestral inference complexed with naringenin (ancR1) | 分子名称: | (4S)-2-METHYL-2,4-PENTANEDIOL, Engineered Chalcone Isomerase ancR1, FORMIC ACID, ... | 著者 | Burke, J.R, Kaltenbach, M, Tawfik, D.S, Noel, J.P. | 登録日 | 2017-07-25 | 公開日 | 2018-05-09 | 最終更新日 | 2023-10-04 | 実験手法 | X-RAY DIFFRACTION (1.5 Å) | 主引用文献 | Evolution of chalcone isomerase from a noncatalytic ancestor. Nat. Chem. Biol., 14, 2018
|
|
5WL5
| Crystal structure of chalcone isomerase engineered from ancestral inference (ancR5) | 分子名称: | CHLORIDE ION, Engineered Chalcone Isomerase ancR5, SULFATE ION | 著者 | Burke, J.R, Kaltenbach, M, Tawfik, D.S, Noel, J.P. | 登録日 | 2017-07-25 | 公開日 | 2018-05-09 | 最終更新日 | 2023-10-04 | 実験手法 | X-RAY DIFFRACTION (1.513 Å) | 主引用文献 | Evolution of chalcone isomerase from a noncatalytic ancestor. Nat. Chem. Biol., 14, 2018
|
|
5WL7
| Crystal structure of chalcone isomerase engineered from ancestral inference (ancCHI*) | 分子名称: | CHLORIDE ION, Engineered Chalcone Isomerase ancCHI* | 著者 | Burke, J.R, Kaltenbach, M, Tawfik, D.S, Noel, J.P. | 登録日 | 2017-07-25 | 公開日 | 2018-05-09 | 最終更新日 | 2024-03-13 | 実験手法 | X-RAY DIFFRACTION (1.9 Å) | 主引用文献 | Evolution of chalcone isomerase from a noncatalytic ancestor. Nat. Chem. Biol., 14, 2018
|
|
4OQI
| Crystal structure of stabilized TEM-1 beta-lactamase variant v.13 carrying R164S/G238S mutations | 分子名称: | CALCIUM ION, SULFATE ION, TEM-94 ES-beta-lactamase | 著者 | Dellus-Gur, E, Elias, M, Fraser, J.S, Tawfik, D.S. | 登録日 | 2014-02-09 | 公開日 | 2015-05-20 | 最終更新日 | 2018-01-17 | 実験手法 | X-RAY DIFFRACTION (1.13 Å) | 主引用文献 | Negative Epistasis and Evolvability in TEM-1 beta-Lactamase--The Thin Line between an Enzyme's Conformational Freedom and Disorder. J. Mol. Biol., 427, 2015
|
|
4OP8
| Room temperature crystal structure of stabilized TEM-1 beta-lactamase variant v.13 carrying G238S mutation | 分子名称: | CALCIUM ION, SULFATE ION, TEM-94 ES-beta-lactamase, ... | 著者 | Dellus-Gur, E, Elias, M, Fraser, J.S, Tawfik, D.S. | 登録日 | 2014-02-05 | 公開日 | 2015-05-20 | 最終更新日 | 2023-09-20 | 実験手法 | X-RAY DIFFRACTION (1.95 Å) | 主引用文献 | Negative Epistasis and Evolvability in TEM-1 beta-Lactamase--The Thin Line between an Enzyme's Conformational Freedom and Disorder. J. Mol. Biol., 427, 2015
|
|
4Q1U
| Serum paraoxonase-1 by directed evolution with the K192Q mutation | 分子名称: | BROMIDE ION, CALCIUM ION, CHLORIDE ION, ... | 著者 | Ben-David, M, Sussman, J.L, Tawfik, D.S. | 登録日 | 2014-04-04 | 公開日 | 2015-02-18 | 最終更新日 | 2023-09-20 | 実験手法 | X-RAY DIFFRACTION (2.302 Å) | 主引用文献 | Catalytic stimulation by restrained active-site floppiness-the case of high density lipoprotein-bound serum paraoxonase-1. J.Mol.Biol., 427, 2015
|
|
4OPY
| Room temperature crystal structure of stabilized TEM-1 beta-lactamase variant v.13 carrying R164S mutation | 分子名称: | CALCIUM ION, Extended spectrum beta-lactamase TEM-63, SULFATE ION, ... | 著者 | Dellus-Gur, E, Elias, M, Fraser, J.S, Tawfik, D.S. | 登録日 | 2014-02-07 | 公開日 | 2015-05-20 | 最終更新日 | 2018-01-17 | 実験手法 | X-RAY DIFFRACTION (1.75 Å) | 主引用文献 | Negative Epistasis and Evolvability in TEM-1 beta-Lactamase--The Thin Line between an Enzyme's Conformational Freedom and Disorder. J. Mol. Biol., 427, 2015
|
|
4OP5
| Crystal structure of stabilized TEM-1 beta-lactamase variant v.13 carrying R164S mutation | 分子名称: | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, CALCIUM ION, Extended spectrum beta-lactamase TEM-63, ... | 著者 | Dellus-Gur, E, Elias, M, Fraser, J.S, Tawfik, D.S. | 登録日 | 2014-02-05 | 公開日 | 2015-05-20 | 最終更新日 | 2023-09-20 | 実験手法 | X-RAY DIFFRACTION (1.05 Å) | 主引用文献 | Negative Epistasis and Evolvability in TEM-1 beta-Lactamase--The Thin Line between an Enzyme's Conformational Freedom and Disorder. J. Mol. Biol., 427, 2015
|
|
4OQH
| Crystal structure of stabilized TEM-1 beta-lactamase variant v.13 carrying R164S mutation in complex with boron-based inhibitor EC25 | 分子名称: | 1,2-ETHANEDIOL, 3-[(2R)-2-{[(2R)-2-amino-2-phenylacetyl]amino}-2-(dihydroxyboranyl)ethyl]benzoic acid, CALCIUM ION, ... | 著者 | Dellus-Gur, E, Elias, M, Fraser, J.S, Tawfik, D.S. | 登録日 | 2014-02-09 | 公開日 | 2015-05-20 | 最終更新日 | 2018-01-17 | 実験手法 | X-RAY DIFFRACTION (1.7 Å) | 主引用文献 | Negative Epistasis and Evolvability in TEM-1 beta-Lactamase--The Thin Line between an Enzyme's Conformational Freedom and Disorder. J. Mol. Biol., 427, 2015
|
|
4OPQ
| Room temperature crystal structure of stabilized TEM-1 beta-lactamase variant v.13 carrying R164S/G238S mutations | 分子名称: | CALCIUM ION, DI(HYDROXYETHYL)ETHER, SULFATE ION, ... | 著者 | Dellus-Gur, E, Elias, M, Fraser, J.S, Tawfik, D.S. | 登録日 | 2014-02-06 | 公開日 | 2015-05-20 | 最終更新日 | 2023-09-20 | 実験手法 | X-RAY DIFFRACTION (1.7 Å) | 主引用文献 | Negative Epistasis and Evolvability in TEM-1 beta-Lactamase--The Thin Line between an Enzyme's Conformational Freedom and Disorder. J. Mol. Biol., 427, 2015
|
|
4OPZ
| Crystal structure of stabilized TEM-1 beta-lactamase variant v.13 carrying G238S mutation in complex with boron-based inhibitor EC25 | 分子名称: | 1,2-ETHANEDIOL, 3-[(2R)-2-{[(2R)-2-amino-2-phenylacetyl]amino}-2-(dihydroxyboranyl)ethyl]benzoic acid, ACETATE ION, ... | 著者 | Dellus-Gur, E, Elias, M, Fraser, J.S, Tawfik, D.S. | 登録日 | 2014-02-07 | 公開日 | 2015-05-20 | 最終更新日 | 2023-09-20 | 実験手法 | X-RAY DIFFRACTION (1.45 Å) | 主引用文献 | Negative Epistasis and Evolvability in TEM-1 beta-Lactamase--The Thin Line between an Enzyme's Conformational Freedom and Disorder. J. Mol. Biol., 427, 2015
|
|
4OPR
| Crystal structure of stabilized TEM-1 beta-lactamase variant v.13 carrying G238S mutation | 分子名称: | CALCIUM ION, SULFATE ION, TEM-94 ES-beta-lactamase, ... | 著者 | Dellus-Gur, E, Elias, M, Fraser, J.S, Tawfik, D.S. | 登録日 | 2014-02-06 | 公開日 | 2015-05-20 | 最終更新日 | 2018-01-17 | 実験手法 | X-RAY DIFFRACTION (1.45 Å) | 主引用文献 | Negative Epistasis and Evolvability in TEM-1 beta-Lactamase--The Thin Line between an Enzyme's Conformational Freedom and Disorder. J. Mol. Biol., 427, 2015
|
|
4OQ0
| Crystal structure of stabilized TEM-1 beta-lactamase variant v.13 carrying R164S/G238S mutation in complex with boron-based inhibitor EC25 | 分子名称: | 1,2-ETHANEDIOL, 3-[(2R)-2-{[(2R)-2-amino-2-phenylacetyl]amino}-2-(dihydroxyboranyl)ethyl]benzoic acid, CALCIUM ION, ... | 著者 | Dellus-Gur, E, Elias, M, Fraser, J.S, Tawfik, D.S. | 登録日 | 2014-02-07 | 公開日 | 2015-05-20 | 最終更新日 | 2018-01-17 | 実験手法 | X-RAY DIFFRACTION (1.42 Å) | 主引用文献 | Negative Epistasis and Evolvability in TEM-1 beta-Lactamase--The Thin Line between an Enzyme's Conformational Freedom and Disorder. J. Mol. Biol., 427, 2015
|
|
4OQG
| Crystal structure of TEM-1 beta-lactamase in complex with boron-based inhibitor EC25 | 分子名称: | 3-[(2R)-2-{[(2R)-2-amino-2-phenylacetyl]amino}-2-(dihydroxyboranyl)ethyl]benzoic acid, Ampicillin resistance protein, ZINC ION | 著者 | Dellus-Gur, E, Elias, M, Fraser, J.S, Tawfik, D.S. | 登録日 | 2014-02-09 | 公開日 | 2015-05-20 | 最終更新日 | 2023-09-20 | 実験手法 | X-RAY DIFFRACTION (2.4 Å) | 主引用文献 | Negative Epistasis and Evolvability in TEM-1 beta-Lactamase--The Thin Line between an Enzyme's Conformational Freedom and Disorder. J. Mol. Biol., 427, 2015
|
|
2RKX
| The 3D structure of chain D, cyclase subunit of imidazoleglycerol_evolvedcerolphosphate synthase | 分子名称: | Cyclase subunit of imidazoleglycerol_evolvedcerolphosphate synthase | 著者 | Tawfik, D, Khersonsky, O, Albeck, S, Dym, O, Israel Structural Proteomics Center (ISPC) | 登録日 | 2007-10-18 | 公開日 | 2008-03-18 | 最終更新日 | 2023-08-30 | 実験手法 | X-RAY DIFFRACTION (2.25 Å) | 主引用文献 | Kemp elimination catalysts by computational enzyme design. Nature, 453, 2008
|
|