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7RDY
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BU of 7rdy by Molmil
SARS-CoV-2 replication-transcription complex bound to nsp13 helicase - nsp13(2)-RTC - engaged class
Descriptor: ADENOSINE-5'-DIPHOSPHATE, ALUMINUM FLUORIDE, CHAPSO, ...
Authors:Chen, J, Malone, B, Campbell, E.A, Darst, S.A.
Deposit date:2021-07-12
Release date:2021-12-01
Last modified:2024-06-05
Method:ELECTRON MICROSCOPY (3.1 Å)
Cite:Ensemble cryo-EM reveals conformational states of the nsp13 helicase in the SARS-CoV-2 helicase replication-transcription complex.
Nat.Struct.Mol.Biol., 29, 2022
7RE0
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BU of 7re0 by Molmil
SARS-CoV-2 replication-transcription complex bound to nsp13 helicase - nsp13(2)-RTC - swiveled class
Descriptor: ADENOSINE-5'-DIPHOSPHATE, ALUMINUM FLUORIDE, Helicase, ...
Authors:Chen, J, Malone, B, Campbell, E.A, Darst, S.A.
Deposit date:2021-07-12
Release date:2021-12-01
Last modified:2024-06-05
Method:ELECTRON MICROSCOPY (3.5 Å)
Cite:Ensemble cryo-EM reveals conformational states of the nsp13 helicase in the SARS-CoV-2 helicase replication-transcription complex.
Nat.Struct.Mol.Biol., 29, 2022
2H27
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BU of 2h27 by Molmil
Crystal Structure of Escherichia coli SigmaE Region 4 Bound to its-35 Element DNA
Descriptor: (4S)-2-METHYL-2,4-PENTANEDIOL, 5'-D(*C*CP*GP*AP*AP*GP*TP*TP*CP*CP*GP*G)-3', 5'-D(*CP*CP*CP*GP*GP*AP*AP*CP*TP*TP*CP*G)-3', ...
Authors:Lane, W.J, Darst, S.A.
Deposit date:2006-05-18
Release date:2006-08-22
Last modified:2024-02-14
Method:X-RAY DIFFRACTION (2.3 Å)
Cite:The structural basis for promoter -35 element recognition by the group IV sigma factors.
Plos Biol., 4, 2006
1SIG
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BU of 1sig by Molmil
CRYSTAL STRUCTURE OF A SIGMA70 SUBUNIT FRAGMENT FROM ESCHERICHIA COLI RNA POLYMERASE
Descriptor: RNA POLYMERASE PRIMARY SIGMA FACTOR
Authors:Malhotra, A, Severinova, E, Darst, S.A.
Deposit date:1997-02-18
Release date:1997-05-15
Last modified:2024-02-14
Method:X-RAY DIFFRACTION (2.6 Å)
Cite:Crystal structure of a sigma 70 subunit fragment from E. coli RNA polymerase.
Cell(Cambridge,Mass.), 87, 1996
3GFK
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BU of 3gfk by Molmil
Crystal structure of Bacillus subtilis Spx/RNA polymerase alpha subunit C-terminal domain complex
Descriptor: DNA-directed RNA polymerase subunit alpha, Regulatory protein spx
Authors:Lamour, V, Westblade, L.F, Campbell, E.A, Darst, S.A.
Deposit date:2009-02-27
Release date:2009-03-10
Last modified:2023-09-06
Method:X-RAY DIFFRACTION (2.3 Å)
Cite:Crystal structure of the in vivo-assembled Bacillus subtilis Spx/RNA polymerase alpha subunit C-terminal domain complex
J.Struct.Biol., 168, 2009
3D36
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BU of 3d36 by Molmil
How to Switch Off a Histidine Kinase: Crystal Structure of Geobacillus stearothermophilus KinB with the Inhibitor Sda
Descriptor: (4S)-2-METHYL-2,4-PENTANEDIOL, ADENOSINE-5'-DIPHOSPHATE, MAGNESIUM ION, ...
Authors:Bick, M.J, Lamour, V, Rajashankar, K.R, Gordiyenko, Y, Robinson, C.V, Darst, S.A.
Deposit date:2008-05-09
Release date:2009-01-13
Last modified:2024-02-21
Method:X-RAY DIFFRACTION (2.03 Å)
Cite:How to switch off a histidine kinase: crystal structure of Geobacillus stearothermophilus KinB with the inhibitor Sda
J.Mol.Biol., 386, 2009
1ZS4
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BU of 1zs4 by Molmil
Structure of bacteriophage lambda cII protein in complex with DNA
Descriptor: DNA - 27mer, Regulatory protein CII
Authors:Jain, D, Kim, Y, Maxwell, K.L, Beasley, S, Gussin, G.N, Edwards, A.M, Darst, S.A.
Deposit date:2005-05-23
Release date:2005-08-23
Last modified:2024-02-14
Method:X-RAY DIFFRACTION (1.7 Å)
Cite:Crystal Structure of Bacteriophage lambdacII and Its DNA Complex.
Mol.Cell, 19, 2005
1YNJ
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BU of 1ynj by Molmil
Taq RNA polymerase-Sorangicin complex
Descriptor: DNA-directed RNA polymerase alpha chain, DNA-directed RNA polymerase beta chain, DNA-directed RNA polymerase beta' chain, ...
Authors:Campbell, E.A, Pavlova, O, Zenkin, N, Leon, F, Irschik, H, Jansen, R, Severinov, K, Darst, S.A.
Deposit date:2005-01-24
Release date:2005-03-15
Last modified:2024-02-14
Method:X-RAY DIFFRACTION (3.2 Å)
Cite:Structural, functional, and genetic analysis of sorangicin inhibition of bacterial RNA polymerase
Embo J., 24, 2005
1ZPQ
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BU of 1zpq by Molmil
STRUCTURE OF BACTERIOPHAGE LAMBDA CII protein
Descriptor: Regulatory protein CII
Authors:Jain, D, Kim, Y, Maxwell, K.L, Beasley, S, Gussin, G.N, Edwards, A.M, Joachimiak, A, Darst, S.A, Midwest Center for Structural Genomics (MCSG)
Deposit date:2005-05-17
Release date:2005-08-23
Last modified:2024-02-14
Method:X-RAY DIFFRACTION (2.8 Å)
Cite:Crystal Structure of Bacteriophage lambdacII and Its DNA Complex.
Mol.Cell, 19, 2005
2P7V
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BU of 2p7v by Molmil
Crystal structure of the Escherichia coli regulator of sigma 70, Rsd, in complex with sigma 70 domain 4
Descriptor: MAGNESIUM ION, RNA polymerase sigma factor rpoD, Regulator of sigma D
Authors:Patikoglou, G.A, Westblade, L.F, Campbell, E.A, Lamour, V, Lane, W.J, Darst, S.A.
Deposit date:2007-03-20
Release date:2007-08-21
Last modified:2024-02-21
Method:X-RAY DIFFRACTION (2.6 Å)
Cite:Crystal structure of the Escherichia coli regulator of sigma70, Rsd, in complex with sigma70 domain 4.
J.Mol.Biol., 372, 2007
1OR7
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BU of 1or7 by Molmil
Crystal Structure of Escherichia coli sigmaE with the Cytoplasmic Domain of its Anti-sigma RseA
Descriptor: RNA polymerase sigma-E factor, Sigma-E factor negative regulatory protein
Authors:Campbell, E.A, Tupy, J.L, Gruber, T.M, Wang, S, Sharp, M.M, Gross, C.A, Darst, S.A.
Deposit date:2003-03-12
Release date:2003-04-15
Last modified:2024-02-14
Method:X-RAY DIFFRACTION (2 Å)
Cite:Crystal structure of Escherichia coli sigmaE with the cytoplasmic domain of its anti-sigma RseA.
Mol.Cell, 11, 2003
2Q1Z
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BU of 2q1z by Molmil
Crystal Structure of Rhodobacter sphaeroides SigE in complex with the anti-sigma ChrR
Descriptor: Anti-Sigma factor ChrR, transcriptional activator ChrR, RpoE, ...
Authors:Campbell, E.A, Darst, S.A.
Deposit date:2007-05-25
Release date:2007-09-18
Last modified:2024-02-21
Method:X-RAY DIFFRACTION (2.4 Å)
Cite:A conserved structural module regulates transcriptional responses to diverse stress signals in bacteria.
Mol.Cell, 27, 2007
1RP3
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BU of 1rp3 by Molmil
Cocrystal structure of the flagellar sigma/anti-sigma complex, Sigma-28/FlgM
Descriptor: RNA polymerase sigma factor SIGMA-28 (FliA), anti sigma factor FlgM
Authors:Sorenson, M.K, Ray, S.S, Darst, S.A.
Deposit date:2003-12-02
Release date:2004-04-06
Last modified:2024-02-14
Method:X-RAY DIFFRACTION (2.3 Å)
Cite:Crystal Structure of the Flagellar Sigma/Anti-Sigma Complex Sigma(28)/FlgM Reveals an Intact Sigma Factor in an Inactive Conformation
Mol.Cell, 14, 2004
1RIO
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BU of 1rio by Molmil
Structure of bacteriophage lambda cI-NTD in complex with sigma-region4 of Thermus aquaticus bound to DNA
Descriptor: (4S)-2-METHYL-2,4-PENTANEDIOL, 27-MER, CALCIUM ION, ...
Authors:Jain, D, Nickels, B.E, Sun, L, Hochschild, A, Darst, S.A.
Deposit date:2003-11-17
Release date:2004-01-27
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (2.3 Å)
Cite:Structure of a ternary transcription activation complex.
Mol.Cell, 13, 2004
6X4Y
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BU of 6x4y by Molmil
Mfd-bound E.coli RNA polymerase elongation complex - IV state
Descriptor: ADENOSINE-5'-DIPHOSPHATE, DNA (64-MER), DNA-directed RNA polymerase subunit alpha, ...
Authors:Llewellyn, E, Chen, J, Kang, J.Y, Darst, S.A.
Deposit date:2020-05-24
Release date:2021-02-03
Last modified:2024-03-06
Method:ELECTRON MICROSCOPY (3.6 Å)
Cite:Structural basis for transcription complex disruption by the Mfd translocase.
Elife, 10, 2021
6X50
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BU of 6x50 by Molmil
Mfd-bound E.coli RNA polymerase elongation complex - V state
Descriptor: ADENOSINE-5'-TRIPHOSPHATE, DNA (64-MER), DNA-directed RNA polymerase subunit alpha, ...
Authors:Llewelyn, E, Chen, J, Kang, J.Y, Darst, S.A.
Deposit date:2020-05-24
Release date:2021-02-03
Last modified:2024-03-06
Method:ELECTRON MICROSCOPY (3.3 Å)
Cite:Structural basis for transcription complex disruption by the Mfd translocase.
Elife, 10, 2021
6X26
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BU of 6x26 by Molmil
Mfd-bound E.coli RNA polymerase elongation complex - L1 state
Descriptor: DNA (64-MER), DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, ...
Authors:Llewellyn, E, Chen, J, Kang, J.Y, Darst, S.A.
Deposit date:2020-05-20
Release date:2021-02-03
Last modified:2024-03-06
Method:ELECTRON MICROSCOPY (4.1 Å)
Cite:Structural basis for transcription complex disruption by the Mfd translocase.
Elife, 10, 2021
6X43
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BU of 6x43 by Molmil
Mfd-bound E.coli RNA polymerase elongation complex - II state
Descriptor: ADENOSINE-5'-TRIPHOSPHATE, DNA (64-MER), DNA-directed RNA polymerase subunit alpha, ...
Authors:Llewellyn, E, Chen, J, Kang, J.Y, Darst, S.A.
Deposit date:2020-05-22
Release date:2021-02-03
Last modified:2024-03-06
Method:ELECTRON MICROSCOPY (3.6 Å)
Cite:Structural basis for transcription complex disruption by the Mfd translocase.
Elife, 10, 2021
4LK0
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BU of 4lk0 by Molmil
Crystal Structure Analysis of the E.coli holoenzyme/T7 Gp2 complex
Descriptor: Bacterial RNA polymerase inhibitor, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, ...
Authors:Bae, B, Darst, S.A.
Deposit date:2013-07-05
Release date:2013-11-13
Last modified:2024-02-28
Method:X-RAY DIFFRACTION (3.9103 Å)
Cite:Phage T7 Gp2 inhibition of Escherichia coli RNA polymerase involves misappropriation of sigma 70 domain 1.1.
Proc.Natl.Acad.Sci.USA, 110, 2013
4LLG
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BU of 4llg by Molmil
Crystal Structure Analysis of the E.coli holoenzyme/Gp2 complex
Descriptor: Bacterial RNA polymerase inhibitor, DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, ...
Authors:Bae, B, Darst, S.A.
Deposit date:2013-07-09
Release date:2013-11-13
Last modified:2024-02-28
Method:X-RAY DIFFRACTION (3.7936 Å)
Cite:Phage T7 Gp2 inhibition of Escherichia coli RNA polymerase involves misappropriation of sigma 70 domain 1.1.
Proc.Natl.Acad.Sci.USA, 110, 2013
4LJZ
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BU of 4ljz by Molmil
Crystal Structure Analysis of the E.coli holoenzyme
Descriptor: DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ...
Authors:Bae, B, Darst, S.A.
Deposit date:2013-07-05
Release date:2013-11-13
Last modified:2024-02-28
Method:X-RAY DIFFRACTION (3.5871 Å)
Cite:Phage T7 Gp2 inhibition of Escherichia coli RNA polymerase involves misappropriation of sigma 70 domain 1.1.
Proc.Natl.Acad.Sci.USA, 110, 2013
4OZ6
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BU of 4oz6 by Molmil
Structure of the Branched Intermediate in Protein Splicing
Descriptor: ALA-MET-ARG-TYR, MAGNESIUM ION, Mxe gyrA intein
Authors:Bick, M.J, Liu, Z, Frutos, S, Vila-Perello, M, Debelouchina, G.T, Darst, S.A, Muir, T.W.
Deposit date:2014-02-14
Release date:2014-05-14
Last modified:2023-09-27
Method:X-RAY DIFFRACTION (2.786 Å)
Cite:Structure of the branched intermediate in protein splicing.
Proc.Natl.Acad.Sci.USA, 111, 2014
4KI2
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BU of 4ki2 by Molmil
Crystallographic analysis of an RNA-polymerase sigma-subunit fragment complexed with -10 promoter element ssDNA
Descriptor: DNA (5'-D(*TP*GP*TP*AP*CP*AP*AP*TP*GP*GP*G)-3'), POTASSIUM ION, RNA polymerase sigma factor
Authors:Feklistov, A, Darst, S.A.
Deposit date:2013-05-01
Release date:2013-09-04
Last modified:2024-02-28
Method:X-RAY DIFFRACTION (3.3 Å)
Cite:Crystallographic analysis of an RNA polymerase sigma-subunit fragment complexed with -10 promoter element ssDNA: quadruplex formation as a possible tool for engineering crystal contacts in protein-ssDNA complexes.
Acta Crystallogr.,Sect.F, 69, 2013
3TBI
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BU of 3tbi by Molmil
Crystal structure of T4 gp33 bound to E. coli RNAP beta-flap domain
Descriptor: DNA-directed RNA polymerase subunit beta, RNA polymerase-associated protein Gp33
Authors:Twist, K.A.F, Campbell, E.A, Darst, S.A.
Deposit date:2011-08-06
Release date:2011-11-23
Method:X-RAY DIFFRACTION (3 Å)
Cite:Crystal structure of T4 gp33 bound to E. coli RNAP beta-flap domain
To be Published
3UGP
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BU of 3ugp by Molmil
Crystal structure of RNA-polymerase sigma subunit domain 2 complexed with -10 promoter element ssDNA oligo (TATAAT)
Descriptor: 5'-D(*TP*GP*TP*AP*TP*AP*AP*TP*GP*GP*G)-3', POTASSIUM ION, RNA polymerase sigma factor
Authors:Feklistov, A, Darst, S.A.
Deposit date:2011-11-02
Release date:2011-12-07
Last modified:2023-09-13
Method:X-RAY DIFFRACTION (2.697 Å)
Cite:Structural basis for promoter-10 element recognition by the bacterial RNA polymerase sigma subunit.
Cell(Cambridge,Mass.), 147, 2011

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