9YHR

AJ09-110 Fab in complex with HIV-1 Env 5MUT-3fill SOSIP

Summary for 9YHR

• Entry DOI: 10.2210/pdb9yhr/pdb
• Related: 9YHO 9YHQ
• EMDB information: 72969 72970 72971
• Descriptor: Transmembrane protein gp41, Envelope glycoprotein gp120, AJ09-110 Fab heavy chain, ... (8 entities in total)
• Functional Keywords: hiv-1, envelope protein, vaccine, immunogen, antibody, bnab, viral protein, viral protein-immune system complex, viral protein/immune system
• Biological source: Human immunodeficiency virus 1 (HIV-1); More
• Total number of polymer chains: 12
• Total formula weight: 310479.43

Authors

Gavor, E.,Gristick, H.B.,Bjorkman, P.J. (deposition date: 2025-09-30, release date: 2026-05-27)

Primary citation

Skelly, A.N.,Gristick, H.B.,Li, H.,Gavor, E.,Connell, A.J.,Kreider, E.F.,Marchitto, L.,Hogarty, M.P.,Newby, M.L.,Allen, J.D.,Liu, W.,West Jr., A.P.,Ayyanathan, K.,Campion, M.S.,Winters, K.,Gordon, C.G.,Osbaldeston, R.A.,Akeley, M.J.,Lewis, E.,Li, Y.,Singh, A.,Cruickshank, K.,Park, Y.,Zhao, C.,Li, X.,Amereh, K.,Van Itallie, E.,Carey, J.W.,Albertus, A.,DeLaitsch, A.T.,Keeffe, J.R.,Lituchy, M.G.,Walsh, A.A.,Morris, D.J.,Habib, R.,Bibollet-Ruche, F.,Mishra, N.,Avillion, G.,Koranda, N.S.,Plante, S.J.,Martella, C.L.,Lora, J.,Wang, E.J.D.,Lewis, M.G.,Martin, M.A.,Nussenzweig, M.C.,Seaman, M.S.,Irvine, D.J.,Wiehe, K.J.,Haynes, B.F.,Wagh, K.,Korber, B.,Andrabi, R.,Crispin, M.,Weissman, D.,Bjorkman, P.J.,Hahn, B.H.,Shaw, G.M.
Induction of broadly neutralizing HIV antibodies by a two-step mechanism informs vaccine design.
Science, :eaec6396-eaec6396, 2026

PubMed Abstract: A major obstacle confronting HIV-1 vaccine and cure research is the lack of an outbred animal model for rapid and consistent induction of broadly neutralizing antibodies (bNAbs). We designed an epitope-focused simian-human immunodeficiency virus (SHIV.5MUT) that elicited broad and potent V3-glycan-targeted antibodies within a year of infection in 14 of 22 macaques compared with 0 of 14 control animals. SHIV.5MUT elicited bNAbs by a two-step mechanism, inducing an initial wave of V1-directed antibodies that selected for Envs with shortened, hypoglycosylated V1 loops, which in turn primed V3-glycan bNAb precursors. Rhesus bNAbs were immunogenetically and structurally diverse, closely resembling human V3-glycan bNAbs. Env-bNAb coevolution revealed a diverse repertoire of bNAb precursors and the Env variants that matured them, yielding a molecular blueprint for vaccine design.

PubMed: 42096521
DOI: 10.1126/science.aec6396

Experimental method

ELECTRON MICROSCOPY (3.1 Å)