9Y0Z

Rubredoxin from Pyrococcus Furiosus at 200K, Alanine-2 N terminus

Summary for 9Y0Z

• Entry DOI: 10.2210/pdb9y0z/pdb
• Related: 9XZU 9Y00 9Y0Y
• Descriptor: Rubredoxin, ZINC ION, POTASSIUM ION, ... (4 entities in total)
• Functional Keywords: iron-sulfur protein, electron transport
• Biological source: Pyrococcus furiosus
• Total number of polymer chains: 1
• Total formula weight: 6136.23

Authors

Doukov, T.,Cramer, S.J.,George, D.,Jenney, F.E. (deposition date: 2025-08-29, release date: 2026-04-29)

Primary citation

Doukov, T.,Leontyev, I.,Jenney Jr., F.E.,George, D.,Cramer, S.P.
Some Like It Hot -Structural Changes in Extremophile Rubredoxin at 120 °C.
Angew.Chem.Int.Ed.Engl., 65:e20302-e20302, 2026

PubMed Abstract: How does the structure of a protein change as the temperature is raised from cryogenic conditions at 100 K to 393 K? Understanding the structure and dynamics of proteins under environmental extremes is relevant for human health, biotechnological applications, and our search for life elsewhere in the universe. Here we reveal the high temperature crystal structure of a hyperthermophilic (Pyrococcus furiosus) rubredoxin at 393 K (120 °C), together with multiple complementary structures down to 100 K. The results are compared with molecular dynamics calculations. Significant changes in H-bonding are observed. Discussions about high-temperature protein structure and stability need to recognize that low temperature structures may not represent the high temperature case.

PubMed: 41287388
DOI: 10.1002/anie.202520302

Experimental method

X-RAY DIFFRACTION (0.86 Å)