9Y0Y
Rubredoxin from Pyrococcus Furiosus at 180K, Alanine-2 N terminus
Summary for 9Y0Y
| Entry DOI | 10.2210/pdb9y0y/pdb |
| Related | 9XZU 9Y00 |
| Descriptor | Rubredoxin, ZINC ION, POTASSIUM ION, ... (4 entities in total) |
| Functional Keywords | iron-sulfur protein, electron transport |
| Biological source | Pyrococcus furiosus |
| Total number of polymer chains | 1 |
| Total formula weight | 6136.23 |
| Authors | Doukov, T.,Cramer, S.J.,George, D.,Jenney, F.E. (deposition date: 2025-08-29, release date: 2026-04-29) |
| Primary citation | Doukov, T.,Leontyev, I.,Jenney Jr., F.E.,George, D.,Cramer, S.P. Some Like It Hot -Structural Changes in Extremophile Rubredoxin at 120 °C. Angew.Chem.Int.Ed.Engl., 65:e20302-e20302, 2026 Cited by PubMed Abstract: How does the structure of a protein change as the temperature is raised from cryogenic conditions at 100 K to 393 K? Understanding the structure and dynamics of proteins under environmental extremes is relevant for human health, biotechnological applications, and our search for life elsewhere in the universe. Here we reveal the high temperature crystal structure of a hyperthermophilic (Pyrococcus furiosus) rubredoxin at 393 K (120 °C), together with multiple complementary structures down to 100 K. The results are compared with molecular dynamics calculations. Significant changes in H-bonding are observed. Discussions about high-temperature protein structure and stability need to recognize that low temperature structures may not represent the high temperature case. PubMed: 41287388DOI: 10.1002/anie.202520302 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (0.852 Å) |
Structure validation
Download full validation report
