9WPE
Cryo-EM structure of the insect sex pheromone receptor ApisOR22-Orco heterocomplex bound with nepetalactone in the closed state.
This is a non-PDB format compatible entry.
Summary for 9WPE
| Entry DOI | 10.2210/pdb9wpe/pdb |
| EMDB information | 66140 |
| Descriptor | Odorant receptor, (4~{a}~{S},7~{S},7~{a}~{R})-4,7-dimethyl-5,6,7,7~{a}-tetrahydro-4~{a}~{H}-cyclopenta[c]pyran-1-one, 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE, ... (4 entities in total) |
| Functional Keywords | pheromone receptor, apo, membrane protein |
| Biological source | Acyrthosiphon pisum (pea aphid) More |
| Total number of polymer chains | 4 |
| Total formula weight | 214164.16 |
| Authors | Dong, Z.,Wang, Y.D.,Guan, Z.Y.,Wang, G.R.,Yin, P. (deposition date: 2025-09-09, release date: 2026-08-26) |
| Primary citation | Dong, Z.,Wang, Y.,Tian, Y.,Guan, Z.,Bai, M.,Zhang, B.,Jia, Z.,Wu, J.,Cao, S.,Gong, Z.,Zhao, X.,Ma, W.,Wang, B.,Wang, G.,Yin, P. Structural basis of sex pheromone detection in aphids. Cell Res., 36:582-594, 2026 Cited by PubMed Abstract: Sex pheromones play a central role in regulating animal behavior and reproduction. In insects, these signals are perceived through specialized odorant receptors (ORs) that mediate species-specific communication and safeguard genetic integrity. However, the structural basis of sex pheromone detection remains largely unresolved. Here, we identified two ORs in the pea aphid Acyrthosiphon pisum, along with the conserved OR co-receptor (Orco), which together mediate recognition of the pheromone components nepetalactone and nepetalactol. Functional assays demonstrated that ApOR21-Orco and ApOR22-Orco specifically respond to nepetalactol and nepetalactone, respectively. Using cryo-electron microscopy, we resolved the structure of the ApOR22-Orco complex in three states - unbound closed, nepetalactone-bound closed, and nepetalactone-bound open - revealing a heterotetrameric ion channel formed by one ApOR22 and three ApOrco subunits. Ligand binding to ApOR22 triggers conformational rearrangements that induce asymmetric pore dilation, thereby enabling ion conduction. Together, these results provide a mechanistic framework for understanding sex pheromone perception in insects and establish a structural foundation for the rational development of environmentally sustainable pest-control strategies. PubMed: 42332065DOI: 10.1038/s41422-026-01267-z PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.1 Å) |
Structure validation
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