9WDX

Structure of SpCas9 with engineered disulfide bonds (R1210C/V1280C and L1119C/P1128C)

Summary for 9WDX

• Entry DOI: 10.2210/pdb9wdx/pdb
• EMDB information: 65909
• Descriptor: CRISPR-associated endonuclease Cas9/Csn1 (1 entity in total)
• Functional Keywords: endonuclease, disulfide engineering, thermostability, hydrolase
• Biological source: Streptococcus pyogenes serotype M1
• Total number of polymer chains: 1
• Total formula weight: 159919.16

Authors

Teo, Z.T.,Ravi, L.R.V.,Jia, X.,Xue, B.,Qu, K.,Yew, W.S. (deposition date: 2025-08-19, release date: 2026-08-26)

• Primary citation: Teo, Z.T.,Hartono, Y.D.,Xue, B.,Jia, X.,Ravi, L.R.V.,Qu, K.,Carstens, C.-P.,Bruhn, L.,Yew, W.S.; Engineering Enhanced Thermostability and High-Temperature Activity in SpCas9 through Strategic C-Terminal Domain Disulfide Bond Incorporation; To Be Published,

Experimental method

ELECTRON MICROSCOPY (3.72 Å)