9WDX

Structure of SpCas9 with engineered disulfide bonds (R1210C/V1280C and L1119C/P1128C)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (P)	CRISPR-associated endonuclease Cas9/Csn1	polymer	1379	159919.2	1	UniProt (Q99ZW2)	Streptococcus pyogenes serotype M1	SpCas9,SpyCas9

Sequence modifications

P: 1 - 1368 (UniProt: Q99ZW2)

PDB	External Database	Details
Cys 1119	Leu 1119	engineered mutation
Cys 1128	Pro 1128	engineered mutation
Cys 1210	Arg 1210	engineered mutation
Cys 1280	Val 1280	engineered mutation
Gly 1369	-	expression tag
Ser 1370	-	expression tag
Ser 1371	-	expression tag
Trp 1372	-	expression tag
Ser 1373	-	expression tag
His 1374	-	expression tag
Pro 1375	-	expression tag
Gln 1376	-	expression tag
Phe 1377	-	expression tag
Glu 1378	-	expression tag
Lys 1379	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	159919.2
All*	Total formula weight	159919.2

*Water molecules are not included.