9VDX
Serial synchrotron crystallography structure of a photosynthetic reaction center using a goniometer-compatible chip-based platform
Summary for 9VDX
| Entry DOI | 10.2210/pdb9vdx/pdb |
| Descriptor | Photosynthetic reaction center cytochrome c subunit, BACTERIOCHLOROPHYLL B, BACTERIOPHEOPHYTIN B, ... (15 entities in total) |
| Functional Keywords | bioenergetics, photosynthesis, serial crystallography, membrane protein |
| Biological source | Blastochloris viridis More |
| Total number of polymer chains | 4 |
| Total formula weight | 145658.03 |
| Authors | Ghosh, S.,Banacore, A.,Neutze, R.,Branden, G. (deposition date: 2025-06-09, release date: 2026-03-25, Last modification date: 2026-04-22) |
| Primary citation | Ghosh, S.,Banacore, A.,Norder, P.,Bjelcic, M.,Kabbinale, A.,Nileshwar, P.,Wehlander, G.,de Sanctis, D.,Basu, S.,Orlans, J.,Vallejos, A.,Chavas, L.M.G.,Neutze, R.,Branden, G. A user-friendly goniometer-compatible fixed-target platform for macromolecular crystallography at synchrotrons. J.Appl.Crystallogr., 59:303-315, 2026 Cited by PubMed Abstract: Fixed-target platforms provide convenient support for microcrystals during serial X-ray crystallography studies using synchrotron radiation. Here, we describe a simple user-friendly 3D-printed support where the crystals are sandwiched between two layers of thin X-ray-transparent membrane resulting in very low scattering background. The platform is compatible with magnetic mounting onto the standard goniometer of macromolecular crystallography beamlines. Our design utilizes a 96-well frame that facilitates hanging-drop experiments directly on the membrane using conventional crystallization plates, thereby eliminating multiple pipetting and crystal handling steps. Crystals can be enclosed in a sandwich and packed into 'cassettes', preventing the risk of the sample drying out during room-temperature transportation to synchrotron sources. The versatility of the platform is demonstrated by five structures solved using different crystallization and data-collection strategies. Lysozyme single-crystal rotational crystallography at room temperature is shown, as well as microcrystal serial data collection under cryogenic conditions. On-chip microcrystallization is illustrated by use of a photosynthetic reaction center as an example. Finally, serial crystallography data collection at room temperature from microcrystals of the membrane protein cytochrome oxidase crystallized in lipidic cubic phase is presented. PubMed: 41959872DOI: 10.1107/S1600576725011513 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.8 Å) |
Structure validation
Download full validation report
