9VDX

Serial synchrotron crystallography structure of a photosynthetic reaction center using a goniometer-compatible chip-based platform

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	293
Detector technology	PIXEL
Collection date	2021-06-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 43 21 2
Unit cell lengths	223.800, 223.800, 113.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.800 - 2.800
Rwork	0.215
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	2.191
Data reduction software	CrystFEL
Phasing software	PHASER
Refinement software	REFMAC (8.0.010)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.000	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	151216	6866
<I/σ(I)>	4.1
Completeness [%]	100.0
Redundancy	412
CC(1/2)	0.936	0.529

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	400 microliter heptane-triol, 20 microliter 1M Potassium Phosphate pH 6.8, 475mg Ammonium Sulphate