9UD1

Cryo-EM structure of STITCHR (R2Tg and Cas9 nickase) bound to guide RNA, template RNA, and target DNA in the target-primed reverse transcription initiation state

Summary for 9UD1

Entry DOI	10.2210/pdb9ud1/pdb
EMDB information	64058
Descriptor	Reverse transcriptase-like protein, THYMIDINE-5'-TRIPHOSPHATE, R2Tg 3'UTR RNA, ... (10 entities in total)
Functional Keywords	retrotransposon, line, reverse transcriptase, endonuclease, rna binding protein, rna binding protein-rna-dna complex, dna binding protein
Biological source	Taeniopygia guttata More
Total number of polymer chains	9
Total formula weight	424315.27
Authors	Komatsu, D.,Hiraizumi, M.,Fujimatsu, K.,Yamada, S.,Yamashita, K.,Nishimasu, H. (deposition date: 2025-04-06, release date: 2026-10-14)
Primary citation	Komatsu, D.,Nishimasu, H. Structure mechanism of the avian R2 retrotransposon for programmable gene integration To Be Published,
Experimental method	ELECTRON MICROSCOPY (3.3 Å)

Structure validation