9UD1
Cryo-EM structure of STITCHR (R2Tg and Cas9 nickase) bound to guide RNA, template RNA, and target DNA in the target-primed reverse transcription initiation state
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Reverse transcriptase-like protein | polymer | 1287 | 146346.7 | 1 | Taeniopygia guttata | ||
| 2 | B (B) | R2Tg 3'UTR RNA | polymer | 78 | 25219.0 | 1 | Taeniopygia guttata | ||
| 3 | C, H (C, H) | DNA non-target strand (bound to R2) | polymer | 60 | 18388.8 | 2 | GenBank (1820520299) | Cloning vector pET-J23100-lacO(SymR+1)-GFP-LVA | |
| 4 | D, I (D, I) | DNA non-target strand (bound to R2) | polymer | 20 | 6273.1 | 2 | Cloning vector pET-J23100-lacO(SymR+1)-GFP-LVA | ||
| 5 | E (E) | DNA non target strand (priming strand bound to R2) | polymer | 41 | 12568.1 | 1 | Cloning vector pET-J23100-lacO(SymR+1)-GFP-LVA | ||
| 6 | F (F) | CRISPR-associated endonuclease Cas9 | polymer | 1367 | 158457.6 | 1 | UniProt (A0A386IRG9) | Streptococcus pyogenes | |
| 7 | G (G) | guide RNA | polymer | 98 | 31607.8 | 1 | Streptococcus pyogenes | ||
| 8 | J, K, L, M (A) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) PubChem (32051) | |||
| 9 | N, O (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) PubChem (888) | |||
| 10 | P (A) | THYMIDINE-5'-TRIPHOSPHATE | non-polymer | 482.2 | 1 | Chemie (TTP) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 423522.9 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 792.4 | |
| All* | Total formula weight | 424315.3 |
