9T5C

Naked mole-rat 80S ribosome in post-translocation non-rotated state

This is a non-PDB format compatible entry.

Summary for 9T5C

• Entry DOI: 10.2210/pdb9t5c/pdb
• EMDB information: 55100
• Descriptor: mRNA, 60S ribosomal protein L7a, Large ribosomal subunit protein uL6, ... (83 entities in total)
• Functional Keywords: naked mole-rat, ribosome, 80s
• Biological source: Heterocephalus glaber (naked mole-rat); More
• Total number of polymer chains: 80
• Total formula weight: 3708321.24

Authors

Gul, M.,Kudryashev, M. (deposition date: 2025-11-05, release date: 2026-06-17, Last modification date: 2026-07-15)

Primary citation

Gul, M.,Rossi, A.,Spahn, C.M.T.,Lewin, G.R.,Kudryashev, M.
Cryo-EM structure of the naked mole-rat ribosome reveals a stabilized split 28S rRNA.
Nat Commun, 17:-, 2026

PubMed Abstract: The naked mole-rat (Heterocephalus glaber) is a long-lived mammal with resistance to cancer and hypoxia, suggesting the evolution of robust proteostasis networks. The ribosome, central for protein synthesis, is key to cellular stress responses and has an unusual feature: the 28S rRNA split; however, the details of its organization remain unknown. Here, we present high-resolution cryo-EM structures of the naked mole-rat 80S ribosome in four states of the elongation cycle. The structures reveal a conserved overall architecture and rRNA modification landscape compared to other mammals, and provide an atomic-level view of the distinct break in the 28S rRNA. This cleavage event, located in the D6 expansion segment, is structurally stabilized by a network of interactions with surrounding ribosomal proteins, maintaining the integrity of the large subunit. Our comparative analysis revealed that this compensatory network preserves a canonical architecture that is nearly indistinguishable from intact mouse and human ribosomes. These findings resolve the structural basis of this distinct cleavage, showing that it is a stable, integrated feature whose function is likely linked to more subtle regulatory mechanisms, rather than inducing major structural rearrangements.

PubMed: 42399278
DOI: 10.1038/s41467-026-75143-0

Experimental method

ELECTRON MICROSCOPY (2.89 Å)