9SYU
shutdown state non-muscle myosin 2A heads region
Summary for 9SYU
| Entry DOI | 10.2210/pdb9syu/pdb |
| EMDB information | 55354 |
| Descriptor | Isoform 1 of Myosin-9, Isoform 1 of Myosin light polypeptide 6, Myosin regulatory light polypeptide 9, ... (6 entities in total) |
| Functional Keywords | myosin, coiled coil, motor protein |
| Biological source | Homo sapiens (human) More |
| Total number of polymer chains | 8 |
| Total formula weight | 982485.55 |
| Authors | Peckham, M.,Carrington, G. (deposition date: 2025-10-14, release date: 2025-10-22, Last modification date: 2026-05-06) |
| Primary citation | Casas-Mao, D.,Carrington, G.,Peckham, M. Cryo-EM structure of shutdown human nonmuscle myosin 2A. Sci Adv, 12:eaed1858-eaed1858, 2026 Cited by PubMed Abstract: Determining the high-resolution structure of the widely expressed nonmuscle myosin 2A (NM2A), in its dephosphorylated shutdown state, is important in understanding its regulation and disease roles. In shutdown molecules, the coiled-coil tail wraps around the myosin heads, preventing them from forming filaments and binding to actin. We have solved the shutdown structure of NM2A to a global resolution of 3.0 angstroms in the head region and 6.3 angstroms for the whole molecule. This reveals specific ionic interactions that explain why the path of the coiled coil and the shutdown mechanism for NM2A differ from those of β-cardiac myosin and provides key insight into how specific mutations likely destabilize the shutdown state, leading to disease. PubMed: 41996489DOI: 10.1126/sciadv.aed1858 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.98 Å) |
Structure validation
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