9S2D
KHNYN exPIN nuclease
Summary for 9S2D
| Entry DOI | 10.2210/pdb9s2d/pdb |
| Descriptor | Protein KHNYN (2 entities in total) |
| Functional Keywords | khnyn, nuclease, antiviral, rna binding protein |
| Biological source | Homo sapiens (human) |
| Total number of polymer chains | 2 |
| Total formula weight | 42378.45 |
| Authors | |
| Primary citation | Youle, R.L.,Lista, M.J.,Brudenell, E.L.,Thompson, B.,Bouton, C.,Morris, E.R.,Neil, S.J.D.,Swanson, C.M.,Taylor, I.A. KHNYN is a manganese-dependent endoribonuclease required for ZAP-mediated antiviral restriction. Nucleic Acids Res., 53:-, 2025 Cited by PubMed Abstract: Zinc finger antiviral protein (ZAP) is a cytoplasmic protein central to host innate immunity to viral infection. ZAP has no intrinsic catalytic activity but inhibits viral replication by binding to CpG dinucleotides in cytoplasmic viral RNA and recruiting other factors to inhibit protein synthesis and target the RNA for degradation. KHNYN is a ZAP-binding protein required for ZAP-restriction of CpG-rich viral genomes. It contains an extended diKH, PIN nuclease, and CUElike domain, each of which are required for ZAP restriction of viral replication. Here, we report a structural, enzymological, and virological study of KHNYN's essential PIN nuclease domain. Our crystal structure reveals an extended PIN domain (ex-PIN) containing a conserved N-terminal arm region required for domain stability and an active site tetra-Asp motif, which are both required for antiviral activity. Unlike the weak activity recently reported for the PIN domain, we demonstrate that the KHNYN ex-PIN domain is a highly active Mn2+-dependent single-stranded RNA endonuclease that cleaves with a preference for ApC, ApA, and UpA dinucleotides. These observations extend our view of KHNYN antiviral activity and suggest an unforeseen role for activation by manganese ions in the ZAP-KHNYN antiviral response. PubMed: 41404804DOI: 10.1093/nar/gkaf1360 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.35 Å) |
Structure validation
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