9RVU

in situ S. pombe hibernating ribosome after 7 days of glucose depletion

This is a non-PDB format compatible entry.

Summary for 9RVU

• Entry DOI: 10.2210/pdb9rvu/pdb
• Related: 9PHC
• EMDB information: 54290
• Descriptor: 18S small subunit ribosomal RNA, Small ribosomal subunit protein eS8B, Small ribosomal subunit protein uS4B, ... (84 entities in total)
• Functional Keywords: ribosome, snor, pombe, yeast, hibernating, glucose, cryo-et, in-situ
• Biological source: Schizosaccharomyces pombe (fission yeast); More
• Total number of polymer chains: 83
• Total formula weight: 3370701.42

Authors

Rosa, H.,Gluc, M.,Jomaa, A.,Mattei, S. (deposition date: 2025-07-09, release date: 2026-04-01, Last modification date: 2026-05-27)

Primary citation

Gluc, M.,Rosa, H.,Bozko, M.,Turner, L.A.,Prince, C.R.,Peskova, Y.,Feaga, H.A.,Gould, K.L.,Mattei, S.,Jomaa, A.
SNOR promotes translation restart after dormancy.
Nature, 2026

PubMed Abstract: Cellular dormancy enables survival during prolonged nutrient limitation by reversibly suppressing protein synthesis. How inactive eukaryotic ribosomes are reactivated when nutrients return remains unclear. Here, using high-resolution in situ cryo-electron tomography in Schizosaccharomyces pombe, we identify SNOR, an SBDS domain-containing ribosome-associated factor that binds at the peptidyl transferase centre and contacts the hypusinated loop of eIF5A during glucose depletion-induced dormancy. Rather than acting as a canonical hibernation factor, SNOR licenses dormant ribosomes for rapid translational restart. Upon glucose repletion, SNOR and eIF5A act together to promote efficient recovery of polysomes and exit from dormancy. These findings define a stress-responsive ribosome restart module that couples carbon-source limitation to surveillance of the ribosomal active site and reactivation of protein synthesis.

PubMed: 42129552
DOI: 10.1038/s41586-026-10530-7

Experimental method

ELECTRON MICROSCOPY (3.38 Å)