9QPQ
The structure of the COPI leaf bound to GOLPH3
Summary for 9QPQ
| Entry DOI | 10.2210/pdb9qpq/pdb |
| EMDB information | 53278 |
| Descriptor | Golgi phosphoprotein 3, Coatomer subunit alpha, Coatomer subunit beta', ... (8 entities in total) |
| Functional Keywords | copi coated vesicles, golph3, golgi transport, vesicular transport, structural protein |
| Biological source | Homo sapiens (human) More |
| Total number of polymer chains | 18 |
| Total formula weight | 1411434.21 |
| Authors | Taylor, R.J.,Tagiltsev, G.,Ciazynska, K.A.,Briggs, J.A.G. (deposition date: 2025-03-28, release date: 2025-10-22) |
| Primary citation | Taylor, R.J.,Zubkov, N.,Ciazynska, K.A.,Kaufman, J.G.G.,Tagiltsev, G.,Owen, D.J.,Briggs, J.A.G.,Munro, S. The mechanistic basis of cargo selection during Golgi maturation. Sci Adv, 11:eaea0016-eaea0016, 2025 Cited by PubMed Abstract: The multiple cisternae of the Golgi apparatus contain resident membrane proteins crucial for lipid and protein glycosylation. How Golgi residents remain in their designated compartments despite a constant flow of secretory cargo is incompletely understood. Here, we determine the structure of the COPI vesicle coat containing GOLPH3, an adaptor protein that binds the cytosolic tails of many Golgi residents. Analysis of this structure, together with structure-guided mutagenesis and functional assays, reveals how GOLPH3 uses coincidence detection of COPI and lipids to engage Golgi residents preferentially at late cisternae. Our findings rationalize the logic of cisternal maturation and explain how COPI can engage different types of substrates in different Golgi cisternae to retrieve some proteins back to the ER while retaining others within the Golgi apparatus. PubMed: 41042862DOI: 10.1126/sciadv.aea0016 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (7.5 Å) |
Structure validation
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