9QPQ
The structure of the COPI leaf bound to GOLPH3
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM CPC |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SUBTOMOGRAM AVERAGING |
| Resolution (Å) | 7.5 |
| Software | subTOM,RELION,NOVACTF,UCSF ChimeraX |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
