9OBN
Crystal structure of malaria transmission-blocking antigen Pfs48/45 C-terminal domain in complex with nanobody B2
Summary for 9OBN
| Entry DOI | 10.2210/pdb9obn/pdb |
| Descriptor | Gametocyte surface protein P45/48, Nanobody B2, SULFATE ION, ... (5 entities in total) |
| Functional Keywords | transmission-blocking, nanobody, 6-cysteine, malaria, protein binding |
| Biological source | Plasmodium falciparum 3D7 More |
| Total number of polymer chains | 8 |
| Total formula weight | 123961.62 |
| Authors | Lyons, F.M.T.,Dietrich, M.H.,Tham, W.H. (deposition date: 2025-04-22, release date: 2026-01-21, Last modification date: 2026-05-27) |
| Primary citation | Lyons, F.M.T.,Chmielewski, J.,Gabriela, M.,Chan, L.J.,Tong, J.,Adair, A.,Zeglinski, K.,Gouil, Q.,Dietrich, M.H.,Tham, W.H. Pfs48/45 nanobodies block Plasmodium falciparum transmission. Plos Pathog., 22:e1013884-e1013884, 2026 Cited by PubMed Abstract: Malaria parasite fertilisation occurs within the Anopheles mosquito midgut. Interventions that inhibit parasite fertilisation prevent ongoing transmission and are important for malaria elimination efforts. Pfs48/45 and Pfs230 are two leading transmission-blocking vaccine candidates. Both proteins form a complex on the surface of sexual stage parasites and are essential for male fertility. Here we have identified nanobodies against Pfs48/45 that recognise gametocytes and have strong transmission-reducing activity. The crystal structure of our most potent nanobody in complex with Pfs48/45 reveals it binds a distinct epitope to TB31F, a leading transmission-blocking monoclonal antibody but to similar epitopes as RUPA-44 and RUPA-117. These results demonstrate the potential of nanobodies as a versatile antibody format that can reduce malaria transmission. PubMed: 41592139DOI: 10.1371/journal.ppat.1013884 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.5 Å) |
Structure validation
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