9LSF

Crystal structure of mRFP1 with a grafted calcium-binding sequence and one bound calcium ion in a calcium-free solution

Summary for 9LSF

• Entry DOI: 10.2210/pdb9lsf/pdb
• Descriptor: Crystal structure of mRFP1 with a grafted calcium-binding sequence, CALCIUM ION, DI(HYDROXYETHYL)ETHER, ... (5 entities in total)
• Functional Keywords: protein engineering, fluorescent protein, mrfp1, calcium-binding sequence
• Biological source: Discosoma
• Total number of polymer chains: 2
• Total formula weight: 63110.08

Authors

Uehara, R.,Kamiya, Y.,Maeda, S.,Okamoto, K.,Toya, S.,Chiba, R.,Amesaka, H.,Takano, K.,Matsumura, H.,Tanaka, S.-i. (deposition date: 2025-02-04, release date: 2025-06-04)

• Primary citation: Uehara, R.,Kamiya, Y.,Maeda, S.,Okamoto, K.,Toya, S.,Chiba, R.,Amesaka, H.,Takano, K.,Matsumura, H.,Tanaka, S.I.; Enhanced secretion through type 1 secretion system by grafting a calcium-binding sequence to modify the folding of cargo proteins; Protein Sci., 34:-, 2025; DOI: 10.1002/pro.70165

Experimental method

X-RAY DIFFRACTION (1.62 Å)