9LSF
Crystal structure of mRFP1 with a grafted calcium-binding sequence and one bound calcium ion in a calcium-free solution
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Red fluorescent protein,grafted calcium-binding sequence | polymer | 280 | 31315.7 | 2 | UniProt (A0A4V4ND72) Pfam (PF01353) | Discosoma | |
| 2 | C, E (A, B) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 3 | D, I (A, B) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 2 | Chemie (PEG) | |||
| 4 | F, G, H (B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 3 | Chemie (EDO) | |||
| 5 | J, K (A, B) | water | water | 18.0 | 296 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 152 (UniProt: A0A4V4ND72)
A, B: 153 - 162 (PDB: 9LSF)
A, B: 163 - 231 (UniProt: A0A4V4ND72)
| PDB | External Database | Details |
|---|---|---|
| Met -36 | - | initiating methionine |
| Gly -35 | - | expression tag |
| Gly -34 | - | expression tag |
| Ser -33 | - | expression tag |
| His -32 | - | expression tag |
| His -31 | - | expression tag |
| His -30 | - | expression tag |
| His -29 | - | expression tag |
| His -28 | - | expression tag |
| His -27 | - | expression tag |
| Gly -26 | - | expression tag |
| Met -25 | - | expression tag |
| Ala -24 | - | expression tag |
| Ser -23 | - | expression tag |
| Met -22 | - | expression tag |
| Thr -21 | - | expression tag |
| Gly -20 | - | expression tag |
| Gly -19 | - | expression tag |
| Gln -18 | - | expression tag |
| Gln -17 | - | expression tag |
| Met -16 | - | expression tag |
| Gly -15 | - | expression tag |
| Arg -14 | - | expression tag |
| Asp -13 | - | expression tag |
| Leu -12 | - | expression tag |
| Tyr -11 | - | expression tag |
| Asp -10 | - | expression tag |
| Asp -9 | - | expression tag |
| Asp -8 | - | expression tag |
| Asp -7 | - | expression tag |
| Lys -6 | - | expression tag |
| Asp -5 | - | expression tag |
| Arg -4 | - | expression tag |
| Trp -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Glu 0 | - | expression tag |
| Leu 1 | - | expression tag |
| Crq 66 | Gln 66 | chromophore |
| Crq 66 | Tyr 67 | chromophore |
| Crq 66 | Gly 68 | chromophore |
A, B: 163 - 231 (UniProt: A0A4V4ND72)
| PDB | External Database | Details |
|---|---|---|
| Gly 232 | - | expression tag |
| Gly 233 | - | expression tag |
| Ser 234 | - | expression tag |
| Gly 235 | - | expression tag |
| Gly 236 | - | expression tag |
| Ser 237 | - | expression tag |
| Glu 238 | - | expression tag |
| Asn 239 | - | expression tag |
| Leu 240 | - | expression tag |
| Tyr 241 | - | expression tag |
| Phe 242 | - | expression tag |
| Gln 243 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 62631.5 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 478.6 | |
| All* | Total formula weight | 63110.1 |
