9LAF

Crystal structure of Elongin BC-EPOP peptide

Summary for 9LAF

• Entry DOI: 10.2210/pdb9laf/pdb
• Descriptor: Elongin-B, Elongin-C, Elongin BC and Polycomb repressive complex 2-associated protein, ... (4 entities in total)
• Functional Keywords: epop, elongin b, elongin c, transcription
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 3
• Total formula weight: 25551.30

Authors

Kim, S.,Lee, B.I. (deposition date: 2025-01-02, release date: 2025-05-21)

Primary citation

Kim, S.,Yeo, H.,Lee, B.I.
Structural analysis of EPOP BC-box binding to the elongin BC complex.
Biochem.Biophys.Res.Commun., 759:151691-151691, 2025

PubMed Abstract: The elongin BC complex (ELOBC) interacts with BC-box-containing proteins and plays a role in various cellular processes, including transcriptional regulation and ubiquitination. Elongin BC and polycomb repressive complex 2-associated protein (EPOP) contains a BC-box motif in its N-terminal region and influences cancer cell proliferation and differentiation. A previous study showed that a BC-box containing an EPOP-derived peptide suppresses cancer cell growth and induces apoptosis by disrupting the interaction between the ELOBC and its partner proteins. Here, we report the crystal structure of the EPOP BC-box peptide bound to the ELOBC and compare it with the structures of other BC-box-containing proteins in complex with the ELOBC. The overall structure of interactions between the BC-box and the ELOC was similar across different complexes, indicating a conserved binding mode. Our structural analysis revealed that the strictly conserved leucine residue (Leu40) within the BC-box of EPOP, which was previously suggested to be critical for interactions between the BC-box and the ELOC, was deeply embedded in the hydrophobic pocket of the ELOC protein. This study provided structural insights into BC-box-mediated protein-protein interactions and may serve as a fundamental resource for developing small molecules that modulate the interactions between ELOBC and BC-box-containing proteins.

PubMed: 40153999
DOI: 10.1016/j.bbrc.2025.151691

Experimental method

X-RAY DIFFRACTION (2.14 Å)