9LAF

Crystal structure of Elongin BC-EPOP peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97942
Spacegroup name	C 2 2 21
Unit cell lengths	120.151, 156.613, 36.817
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.880 - 2.140
R-factor	0.1964
Rwork	0.193
R-free	0.23080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.800
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.190
High resolution limit [Å]	2.140	2.150
Rmerge	0.094
Number of reflections	19316	788
<I/σ(I)>	16.25
Completeness [%]	98.4
Redundancy	5.7
CC(1/2)	0.995	0.878

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	15% v/v 2-Propanol, 0.1 M Sodium citrate tribasic dihydrate pH 5.0, 10% w/v Polyethylene glycol 10,000