9L9O
Cryo-EM structure of apo GPR50 with BRIL fusion, anti-BRIL Fab, and anti-Fab Nb complex
Summary for 9L9O
| Entry DOI | 10.2210/pdb9l9o/pdb |
| EMDB information | 62911 |
| Descriptor | anti-Fab Nb, anti-BRIL Fab Light Chain, anti-BRIL Fab Heavy Chain, ... (4 entities in total) |
| Functional Keywords | g-protein coupled receptor, membrane protein, membrane protein-immune system complex, membrane protein/immune system |
| Biological source | synthetic construct More |
| Total number of polymer chains | 4 |
| Total formula weight | 134282.45 |
| Authors | |
| Primary citation | Shin, J.,Baek, D.,Kim, J.,Park, J.,Jeong, E.,Kim, Y.,Kim, Y.J.,Cho, Y. Structure of the melatonin-related orphan receptor, GPR50. Mol.Cells, :100331-100331, 2026 Cited by PubMed Abstract: GPR50 is an orphan GPCR that belongs to the member of melatonin-related receptor family. GPR50 plays roles in various physiological processes, including cancer progression, Notch signaling, and insulin, leptin, and glucocorticoid signaling. GPR50 forms a complex with melatonin receptor type 1A or 1B, and regulates signaling activity of melatonin receptor type 1A. Although endogenous agonists have not been characterized, GPR50 may have its own signaling activity, which is undefined at present. In this study, in an attempt to characterize the orphan activity of GPR50, we determined the 3.4 Å structure of ligand-free GPR50 using cryo-electron microscopy. We showed that GPR50 exhibits moderate constitutive activity through interaction with Gα. The structure reveals a putative ligand binding pocket and ligand access channels of GPR50 that differ from those of melatonin receptors. Based on the comparison with the AlphaFold3-predicted active state, we propose an activation mechanism of GPR50. Our findings could serve as a platform in identifying the synthetic or endogenous ligands of GPR50, providing insights into the elusive G-protein-dependent signaling of GPR50. PubMed: 41666959DOI: 10.1016/j.mocell.2026.100331 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.98 Å) |
Structure validation
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