9L9O

Cryo-EM structure of apo GPR50 with BRIL fusion, anti-BRIL Fab, and anti-Fab Nb complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (D)	anti-Fab Nb	polymer	162	17727.3	1		synthetic construct
2	B (C)	anti-BRIL Fab Light Chain	polymer	237	25638.7	1		Homo sapiens
3	C (B)	anti-BRIL Fab Heavy Chain	polymer	289	31174.8	1		Homo sapiens
4	D (A)	Melatonin-related receptor,Soluble cytochrome b562	polymer	533	59741.7	1	UniProt (Q13585) UniProt (P0ABE7)	Homo sapiens (human)	G protein-coupled receptor 50,H9,Cytochrome b-562

Sequence modifications

A: 1 - 219 (UniProt: Q13585)

PDB	External Database	Details
Met -24	-	initiating methionine
Arg -23	-	expression tag
Leu -22	-	expression tag
Leu -21	-	expression tag
Thr -20	-	expression tag
Ala -19	-	expression tag
Leu -18	-	expression tag
Phe -17	-	expression tag
Ala -16	-	expression tag
Tyr -15	-	expression tag
Phe -14	-	expression tag
Ile -13	-	expression tag
Val -12	-	expression tag
Ala -11	-	expression tag
Leu -10	-	expression tag
Ile -9	-	expression tag
Leu -8	-	expression tag
Ala -7	-	expression tag
Phe -6	-	expression tag
Ser -5	-	expression tag
Val -4	-	expression tag
Ser -3	-	expression tag
Ala -2	-	expression tag
Lys -1	-	expression tag
Ser 0	-	expression tag

A: 1004 - 1109 (UniProt: P0ABE7)

PDB	External Database	Details
Trp 1010	Met 29	engineered mutation
Ile 1105	His 124	engineered mutation
Leu 1109	Arg 128	engineered mutation
Glu 1110	-	linker
Arg 1111	-	linker
Ala 1112	-	linker
Arg 1113	-	linker
Ser 1114	-	linker
Thr 1115	-	linker
Leu 1116	-	linker

A: 227 - 355 (UniProt: Q13585)

PDB	External Database	Details
Arg 1001	-	linker
Gln 1002	-	linker
Leu 1003	-	linker
Arg 356	-	expression tag
Gly 357	-	expression tag
Arg 358	-	expression tag
Gly 359	-	expression tag
Gly 360	-	expression tag
Ser 361	-	expression tag
Leu 362	-	expression tag
Glu 363	-	expression tag
Val 364	-	expression tag
Leu 365	-	expression tag
Phe 366	-	expression tag
Gln 367	-	expression tag
Gly 368	-	expression tag
Pro 369	-	expression tag
Gly 370	-	expression tag
Ser 371	-	expression tag
Trp 372	-	expression tag
Ser 373	-	expression tag
His 374	-	expression tag
Pro 375	-	expression tag
Gln 376	-	expression tag
Phe 377	-	expression tag
Glu 378	-	expression tag
Lys 379	-	expression tag
Gly 380	-	expression tag
Gly 381	-	expression tag
Gly 382	-	expression tag
Ser 383	-	expression tag
Gly 384	-	expression tag
Gly 385	-	expression tag
Gly 386	-	expression tag
Ser 387	-	expression tag
Gly 388	-	expression tag
Gly 389	-	expression tag
Ser 390	-	expression tag
Ala 391	-	expression tag
Trp 392	-	expression tag
Ser 393	-	expression tag
His 394	-	expression tag
Pro 395	-	expression tag
Gln 396	-	expression tag
Phe 397	-	expression tag
Glu 398	-	expression tag
Lys 399	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	134282.4
All*	Total formula weight	134282.4

*Water molecules are not included.