9L2O

Cryo-EM structure and rational engineering of a novel efficient ochratoxin A-detoxifying amidohydrolase

Summary for 9L2O

• Entry DOI: 10.2210/pdb9l2o/pdb
• EMDB information: 62778
• Descriptor: Imidazolonepropionase, ZINC ION, (2~{S})-2-[[(3~{R})-5-chloranyl-3-methyl-8-oxidanyl-1-oxidanylidene-3,4-dihydroisochromen-7-yl]carbonylamino]-3-phenyl-propanoic acid (3 entities in total)
• Functional Keywords: amide hydrolase, iron-binding, ochratoxin a, zn, hydrolase
• Biological source: Pseudoxanthomonas wuyuanensis
• Total number of polymer chains: 8
• Total formula weight: 352131.61

Authors

Dai, L.H.,Xu, Y.H.,Hu, Y.M.,He, B.Y.,Huang, J.P.,Xie, Z.Z.,Li, H.,Niu, D.,Guo, R.-T.,Chen, C.-C. (deposition date: 2024-12-17, release date: 2026-05-06)

Primary citation

Hu, Y.,Xu, Y.,He, B.,Niu, D.,Yang, X.,Huang, J.,Xie, Z.,Shen, P.,Li, X.,Bai, M.,Liu, Z.,Li, H.,Yang, Y.,Huang, J.W.,Chen, C.C.,Guo, R.T.,Dai, L.
Structure-guided protein engineering and immobilization of an amidohydrolase for efficient ochratoxin A detoxification.
Int.J.Biol.Macromol., 358:151671-151671, 2026

PubMed Abstract: Ochratoxin A (OTA) is a pervasive and significant mycotoxin that poses serious health risks to humans and animals. The development of efficient biocatalysts for the enzymatic detoxification of OTA is of great importance. In this study, we enhanced the OTA-degrading activity of three amidohydrolases (ADHs) by up to ninefold. This improvement was achieved by reducing steric hindrance in the binding pocket and fine-tuning the hydrophilic interactions between the enzyme and substrate. The most efficient variant, PwADH/DM, was immobilized onto magnetic FeO nanoparticles functionalized by the co-deposition of dopamine and polyethyleneimine. Under optimal conditions, this immobilization process achieved a high immobilization efficiency (85.4%) and activity recovery (76.9%). The immobilized enzyme exhibited enhanced pH stability and thermostability, along with good storage stability, reusability, and recyclability. More importantly, the immobilized enzyme completely degraded 100 ng/mL OTA in contaminated milk without affecting the milk's properties. These findings expand our understanding of the molecular mechanisms governing substrate binding and catalysis in OTA-degrading ADHs. Furthermore, they provide a blueprint for enzyme-based OTA decontamination during food processing.

PubMed: 41903634
DOI: 10.1016/j.ijbiomac.2026.151671

Experimental method

ELECTRON MICROSCOPY (2.86 Å)