9KL3

CryoEM structure of Pleurocybella porrigens lectin (PPL) in complex with GalNAc

Summary for 9KL3

• Entry DOI: 10.2210/pdb9kl3/pdb
• EMDB information: 62399
• Descriptor: Lectin, 2-acetamido-2-deoxy-alpha-D-galactopyranose (2 entities in total)
• Functional Keywords: trefoil, lectin, sugar binding protein
• Biological source: Pleurocybella porrigens
• Total number of polymer chains: 1
• Total formula weight: 14846.33

Authors

Ishimoto, N.,Adachi, D.,Kawabata, H.,Park, S.Y.,Tame, J.R.H.,Kamata, K. (deposition date: 2024-11-14, release date: 2025-11-19, Last modification date: 2025-12-31)

Primary citation

Adachi, D.,Ishimoto, N.,Mizutani, K.,Takahashi, K.,Kubota, R.,Kawabata, H.,Park, S.Y.,Vandebroek, L.,Voet, A.R.D.,Yamada, M.,Ozeki, Y.,Fujii, Y.,Fujita, H.,Tame, J.R.H.,Kamata, K.
Crystal and Cryo-EM structure of PPL, a novel hexameric R-type lectin from the poisonous mushroom Pleurocybella porrigens.
Glycobiology, 36:-, 2025

PubMed Abstract: Pleurocybella porrigens is a mushroom that grows widely around the temperate northern hemisphere, and was once considered edible, especially in Japan. Following a number of deaths in 2004, investigations revealed the presence of various toxins, including a lectin (PPL) that apparently survives cooking and enters the bloodstream via the stomach. We have cloned PPL and solved its structure by X-ray crystallography and cryo-EM. We report the sugar binding properties of this β-trefoil lectin, which has a novel hexameric structure.

PubMed: 41342409
DOI: 10.1093/glycob/cwaf082

Experimental method

ELECTRON MICROSCOPY (3.24 Å)