9KL3
CryoEM structure of Pleurocybella porrigens lectin (PPL) in complex with GalNAc
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TALOS ARCTICA |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.24 |
| Number of Particle | 150096 |
| Software | Topaz,cryoSPARC,SerialEM,PHENIX,Coot,UCSF ChimeraX |
| Symmetry | D3 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
