9J4N
Crystal structure of E. coli Leucine tRNA with UAG anticodon
Summary for 9J4N
| Entry DOI | 10.2210/pdb9j4n/pdb |
| Descriptor | Leucine tRNA_UAG (85-MER) (1 entity in total) |
| Functional Keywords | trna, rna |
| Biological source | Escherichia coli K-12 |
| Total number of polymer chains | 1 |
| Total formula weight | 27441.31 |
| Authors | |
| Primary citation | Cho, G.,Yoo, J.,Shin, K.,Lee, J.,Lim, J.,Kim, J. Crystal structures of free-state tRNALeu reveal conformational flexibility of Type-II tRNAs. Rna, 2025 Cited by PubMed Abstract: Transfer RNAs (tRNAs) are classified into Type-I and Type-II based on the length of their variable loops, with Type-II characterized by an extended variable loop. While structures of Type-I tRNAs have been well-documented, standalone structures of Type-II tRNAs have not been reported. Here, we present the first crystal structures of two free-state Type-II tRNAs, specifically tRNALeu from Bacillus subtilis and Escherichia coli. Our structures reveal that the B. subtilis tRNALeu anticodon stem-loop (ASL) retains its canonical conformation. The variable loops in both structures are well-defined, displaying a distinctive tetranucleotide loop conformation. Comparisons with Type-I tRNA and biomolecule-bound tRNAs highlight the flexibility of the ASL, variable loop, and terminal CCA residues in Type-II tRNAs, suggesting that this structural plasticity is crucial for their biological interactions and function. These findings provide new insights into the structural dynamics and functional roles of Type-II tRNAs. PubMed: 40562537DOI: 10.1261/rna.080596.125 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.9 Å) |
Structure validation
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