9J4N

Crystal structure of E. coli Leucine tRNA with UAG anticodon

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2023-04-13
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 21 21 2
Unit cell lengths	61.365, 114.179, 53.817
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.681 - 2.900
R-factor	0.24762
Rwork	0.246
R-free	0.26961
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.680
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.681	3.331
High resolution limit [Å]	2.897	2.897
Rmerge	0.191	1.750
Rmeas	0.200	1.821
Rpim	0.059	0.498
Number of reflections	4225	352
<I/σ(I)>	11.1	1.8
Completeness [%]	86.8	61.7
Redundancy	11.5	13.2
CC(1/2)	0.997	0.626

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	297	0.25 M ammonium sulfate, 0.1 M sodium acetate trihydrate, pH 4.6, 32% (w/v) polyethylene glycol monomethyl ether 2000