9IQT

structure of niacin-HCA2-Gi

Summary for 9IQT

• Entry DOI: 10.2210/pdb9iqt/pdb
• EMDB information: 60795
• Descriptor: Hydroxycarboxylic acid receptor 2, Guanine nucleotide-binding protein G(i) subunit alpha-1, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, ... (6 entities in total)
• Functional Keywords: agonist, complex, membrane protein/immune system, membrane protein-immune system complex
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 5
• Total formula weight: 152703.28

Authors

Liu, Y.,Zhou, Z. (deposition date: 2024-07-13, release date: 2024-10-02, Last modification date: 2024-11-13)

Primary citation

Liu, Y.,Zhou, Z.,Guan, F.,Han, Z.,Zhu, C.,Ye, S.,Yu, X.,Qiao, A.
Ligand Recognition and Activation Mechanism of the Alicarboxylic Acid Receptors.
J.Mol.Biol., 436:168795-168795, 2024

PubMed Abstract: Endogenous ligands for alicarboxylic acid receptors are important metabolic intermediates that play a significant role in regulating body energy and maintaining homeostasis. However, the molecular mechanism of alicarboxylate ligand-mediated counterpart receptors is currently unclear. We resolve the active state structure of HCA2-niacin, and the structural analysis explains the mechanism of niacin selectivity in the alicarboxylic acid receptors family. Homology modeling, molecular dynamics simulation and mutagenesis experiments reveal different ligand recognition modes and activation mechanisms of the alicarboxylic acid receptors, analyze the flexibility of the binding pocket and elucidate the important role of disulfide bonds on receptor activation and ligand binding. These more detailed molecular mechanisms further elucidate the relevant mechanisms of human metabolism and provide key clues for subsequent drug development of alicarboxylic acid receptors.

PubMed: 39299383
DOI: 10.1016/j.jmb.2024.168795

Experimental method

ELECTRON MICROSCOPY (2.9 Å)