9H74
Late-stage 48S Initiation Complex with eIF3 (LS48S-eIF3 IC) guided by the trans-RNA
This is a non-PDB format compatible entry.
Summary for 9H74
| Entry DOI | 10.2210/pdb9h74/pdb |
| EMDB information | 51911 |
| Descriptor | Initiator methionylated tRNA, 40S ribosomal protein S4, Ribosomal protein S5, ... (52 entities in total) |
| Functional Keywords | 48s late-stage initiation complex, programmable mrna translation initiation, trans-rna, activation of mrna translation, translation |
| Biological source | Oryctolagus cuniculus More |
| Total number of polymer chains | 51 |
| Total formula weight | 2140721.30 |
| Authors | Nguyen, T.T.,Hashem, Y.,Rocha, R.E.O.,Boissier, F.,Qian, S.B.,Jia, L.,Uematsu, S.,Gu, Y.,Shi, S. (deposition date: 2024-10-25, release date: 2025-12-10) |
| Primary citation | Jia, L.,Nguyen, T.T.,Uematsu, S.,Gu, Y.,Shi, S.,Hashem, Y.,Qian, S.B. Programmable initiation of mRNA translation by trans-RNA. Nat.Biotechnol., 2025 Cited by PubMed Abstract: Several approaches exist to silence genes, but few tools are available to activate individual mRNAs for translation inside cells. Guiding ribosomes to specific start codons without altering the original sequence remains a formidable task. Here we design capped trans-RNAs capable of directing ribosomes to specific initiation sites on individual mRNAs when the trans-cap is positioned near the target start codon. Structural and biochemical data suggest that the capped trans-RNA facilitates ribosome loading and scanning on the target mRNA through a synergistic mechanism involving alternative cap recognition. The trans-RNA also acts independently of the cap on the target mRNA, enabling translation of circular RNAs lacking internal ribosome entry sites. We apply trans-RNAs in vivo to achieve programmable alternative translation of endogenous genes in mouse liver. Finally, we provide the evidence for the existence of natural transcripts that, similarly to exogenous trans-RNAs, activate translation of endogenous mRNAs. PubMed: 41272315DOI: 10.1038/s41587-025-02897-1 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.9 Å) |
Structure validation
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