9GLC
NONO/SFPQ filament: local refinement central units (strand 1)
This is a non-PDB format compatible entry.
Summary for 9GLC
| Entry DOI | 10.2210/pdb9glc/pdb |
| Related | 9GLD 9GNI |
| EMDB information | 51438 51471 |
| Descriptor | Splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated), Non-POU domain containing octamer binding (2 entities in total) |
| Functional Keywords | filament, rna binding, dna binding, gene regulation, nuclear protein |
| Biological source | Cricetulus griseus (Chinese hamster) More |
| Total number of polymer chains | 10 |
| Total formula weight | 673360.25 |
| Authors | Rasmussen, T.,Bottcher, B. (deposition date: 2024-08-27, release date: 2025-09-10, Last modification date: 2026-03-11) |
| Primary citation | Rasmussen, T.,Kuspert, J.,Schonemann, L.,Geiger, D.,Bottcher, B. The gene-regulating proteins NONO and SFPQ assemble into ordered filaments. Commun Biol, 9:117-117, 2025 Cited by PubMed Abstract: Proteins of the Drosophila behaviour/human splicing (DBHS) family are involved in many aspects of gene regulation and maintenance like transcription, splicing and DNA repair. DBHS proteins form obligate homo- and heterodimers through interactions within a globular domain and can further dynamically oligomerise through α-helical coiled-coils, which is crucial for many functions. While the atomic structures of the dimers are established, the arrangement in higher oligomers is unknown. Here we present the structure of a filamentous NONO/SFPQ heterooligomer resolved by cryo-EM. The filaments form a double helix which is stabilized by an interdigitating network of coiled-coil interactions. PubMed: 41476260DOI: 10.1038/s42003-025-09396-8 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.3 Å) |
Structure validation
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