9GDP

Cryo-EM structure of Vibrio cholerae RNA polymerase holoenzyme bound to an ompU promoter DNA fragment and 5-mer RNA

Summary for 9GDP

• Entry DOI: 10.2210/pdb9gdp/pdb
• EMDB information: 51274
• Descriptor: DNA-directed RNA polymerase subunit beta, MAGNESIUM ION, DNA-directed RNA polymerase subunit beta', ... (10 entities in total)
• Functional Keywords: rna polymerase, open promoter complex, ompu, vibrio cholerae, transcription
• Biological source: Vibrio cholerae O395; More
• Total number of polymer chains: 10
• Total formula weight: 539321.64

Authors

Alcaide-Jimenez, A.,Baudin, F.,Canals, A.,Machon, C.,Murciano, B.,Fabrega-Ferrer, M.,Bantysh, O.,Perez-Luque, R.,Krukonis, E.S.,Muller, C.W.,Coll, M. (deposition date: 2024-08-06, release date: 2025-12-17)

• Primary citation: Alcaide-Jimenez, A.,Canals, A.,Baudin, F.,Machon, C.,Fabrega-Ferrer, M.,Bantysh, O.,Perez-Luque, R.,Murciano, B.,Mohammad, A.A.,Rowse, M.J.,Ferracciolo, J.M.,Krukonis, E.S.,Muller, C.W.,Coll, M.; Structures of Vibrio cholerae transcription complexes reveal how ToxR and TcpP recruit the RNA polymerase and activate virulence genes; Science Advances, 2025

Experimental method

ELECTRON MICROSCOPY (2.6 Å)