9GDP
Cryo-EM structure of Vibrio cholerae RNA polymerase holoenzyme bound to an ompU promoter DNA fragment and 5-mer RNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (C) | DNA-directed RNA polymerase subunit beta | polymer | 1341 | 149696.5 | 1 | UniProt (A5F3P5) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) | Vibrio cholerae O395 | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 2 | B (D) | DNA-directed RNA polymerase subunit beta' | polymer | 1401 | 155234.3 | 1 | UniProt (A5F3P6) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Vibrio cholerae O395 | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 3 | C (E) | DNA-directed RNA polymerase subunit omega | polymer | 90 | 10068.4 | 1 | UniProt (A5F4S8) Pfam (PF01192) | Vibrio cholerae O395 | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 4 | D, E, H (A, B, G) | DNA-directed RNA polymerase subunit alpha | polymer | 330 | 36461.5 | 3 | UniProt (A5F572) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Vibrio cholerae O395 | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 5 | F (F) | RNA polymerase sigma factor RpoD | polymer | 621 | 70749.6 | 1 | UniProt (A0A0H3AJ64) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) | Vibrio cholerae O395 | Sigma-70 |
| 6 | G (H) | RNA 5-mer (5'-AAUAG-3') | polymer | 5 | 1594.0 | 1 | Vibrio cholerae O395 | ||
| 7 | I (T) | ompU promoter non-template DNA strand | polymer | 69 | 21329.7 | 1 | Vibrio cholerae O395 | ||
| 8 | J (L) | ompU promoter template DNA strand | polymer | 69 | 21109.6 | 1 | GenBank (657184927) | Vibrio cholerae O395 | |
| 9 | K, L (D) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 10 | M (D) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 539166.5 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 155.1 | |
| All* | Total formula weight | 539321.6 |
