9FJ3

Structure of ubiquitin bound of coiled-coil UIM form 2

This is a non-PDB format compatible entry.

Summary for 9FJ3

• Entry DOI: 10.2210/pdb9fj3/pdb
• Descriptor: Polyubiquitin-C, GLU-GLN-GLU-ILE-GLU-GLU-LEU-GLU-ILE-GLU-ILE-ALA-ILE-LEU-LEU-SER-GLU-ILE-GLU-GLY, LYS-GLN-LYS-ILE-ALA-ALA-LEU-LYS-TYR-LYS-ILE-ALA-ALA-LEU-LYS-GLN-LYS-ILE, ... (5 entities in total)
• Functional Keywords: ubiquitin, coiled-coil peptide, uim, signaling protein
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 6
• Total formula weight: 27337.27

Authors

Paredes Vergara, P.,Huang, D.T. (deposition date: 2024-05-30, release date: 2024-09-18, Last modification date: 2024-10-16)

Primary citation

Vosbein, P.,Vergara, P.P.,Huang, D.T.,Thomson, A.R.
An engineered ubiquitin binding coiled coil peptide.
Chem Sci, 15:15776-15782, 2024

PubMed Abstract: Recognition of ubiquitin (Ub) is often mediated by small Ub binding domains such as the Ubiquitin Interacting Motif (UIM). Most Ub binding events are low affinity interactions, and designing stronger binders for Ub can be challenging. We here report the design of a short crosslinked coiled coil (CC) which is conformationally and chemically stable, and which can act as a scaffold to present the key binding residues from known UIM sequences. Doing so gives rise to a hybrid CC peptide that reconciles the important features of both UIM and CC sequences. We show by fluorescence polarization assays that this crosslinked 'CC-UIM' peptide exhibits enhanced binding to Ub compared to the original UIM sequence. Furthermore, we report a crystal structure of this peptide in complex with Ub. These studies show that preorganization of a small number of important binding residues onto a stable helical scaffold can be a successful strategy for binder design.

PubMed: 39268210
DOI: 10.1039/d4sc04204b

Experimental method

X-RAY DIFFRACTION (1.4 Å)