9FJ3
Structure of ubiquitin bound of coiled-coil UIM form 2
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-08-02 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.95374 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.450, 50.090, 52.200 |
| Unit cell angles | 90.00, 101.26, 90.00 |
Refinement procedure
| Resolution | 41.630 - 1.400 |
| R-factor | 0.2179 |
| Rwork | 0.217 |
| R-free | 0.23770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.721 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.630 | 1.440 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 58645 | 3872 |
| <I/σ(I)> | 10.5 | |
| Completeness [%] | 97.0 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.999 | 0.781 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.1 M amino acids, 0.1 M buffer system 1, pH 6.6-6.7 and 36-38 %(v/v) precipitant mix 1 (Molecular Dimension) |
