9F95
Complex of phenazine biosynthesis enzyme PhzF with 2-amino-3-hydroxy-5-(3-hydroxyphenyl)benzoic acid
This is a non-PDB format compatible entry.
Summary for 9F95
| Entry DOI | 10.2210/pdb9f95/pdb |
| Related | 9F92 9F93 9F94 9F96 |
| Descriptor | Trans-2,3-dihydro-3-hydroxyanthranilate isomerase, 2-azanyl-5-(3-hydroxyphenyl)-3-oxidanyl-benzoic acid (3 entities in total) |
| Functional Keywords | inhibitor, complex, phenazine biosynthesis, pyocyanin, isomerase |
| Biological source | Pseudomonas fluorescens |
| Total number of polymer chains | 1 |
| Total formula weight | 32501.79 |
| Authors | Baumgarten, J.,Schneider, P.,Blankenfeldt, W.,Kunick, C. (deposition date: 2024-05-07, release date: 2024-10-09) |
| Primary citation | Baumgarten, J.,Schneider, P.,Thiemann, M.,Zimmermann, M.,Diederich, C.,Blankenfeldt, W.,Kunick, C. Substrate-Based Ligand Design for Phenazine Biosynthesis Enzyme PhzF. Chemmedchem, :e202400466-e202400466, 2024 Cited by PubMed Abstract: The phenazine pyocyanin is an important virulence factor of the pathogen Pseudomonas aeruginosa, which is on the WHO list of antibiotic resistant "priority pathogens". In this study the isomerase PhzF, a key bacterial enzyme of the pyocyanin biosynthetic pathway, was investigated as a pathoblocker target. The aim of the pathoblocker strategy is to reduce the virulence of the pathogen without killing it, thus preventing the rapid development of resistance. Based on crystal structures of PhzF, derivatives of the inhibitor 3-hydroxyanthranilic acid were designed. Co-crystal structures of the synthesized derivatives with PhzF revealed spacial limitations of the binding pocket of PhzF in the closed conformation. In contrast, ligands aligned to the open conformation of PhzF provided more room for structural modifications. The intrinsic fluorescence of small 3-hydroxyanthranilic acid derivatives enabled direct affinity determinations using FRET assays. The analysis of structure-activity relationships showed that the carboxylic acid moiety is essential for binding to the target enzyme. The results of this study provide fundamental structural insights that will be useful for the design of PhzF-inhibitors. PubMed: 39163032DOI: 10.1002/cmdc.202400466 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.46 Å) |
Structure validation
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