9F5Y

Structure of the Chlamydomonas reinhardtii respiratory complex I from respiratory supercomplex

Summary for 9F5Y

• Entry DOI: 10.2210/pdb9f5y/pdb
• EMDB information: 50203
• Descriptor: NADH:ubiquinone oxidoreductase 24 kD subunit, Acyl carrier protein, NADH:ubiquinone oxidoreductase B14 subunit, ... (64 entities in total)
• Functional Keywords: mitochondria, respiratory complex, respiration, alga, membrane protein
• Biological source: Chlamydomonas reinhardtii; More
• Total number of polymer chains: 51
• Total formula weight: 1181718.57

Authors

Waltz, F.,Righetto, R.,Kotecha, A.,Engel, B.D. (deposition date: 2024-04-30, release date: 2025-03-12, Last modification date: 2025-04-02)

Primary citation

Waltz, F.,Righetto, R.D.,Lamm, L.,Salinas-Giege, T.,Kelley, R.,Zhang, X.,Obr, M.,Khavnekar, S.,Kotecha, A.,Engel, B.D.
In-cell architecture of the mitochondrial respiratory chain.
Science, 387:1296-1301, 2025

PubMed Abstract: Mitochondria regenerate adenosine triphosphate (ATP) through oxidative phosphorylation. This process is carried out by five membrane-bound complexes collectively known as the respiratory chain, working in concert to transfer electrons and pump protons. The precise organization of these complexes in native cells is debated. We used in situ cryo-electron tomography to visualize the native structures and organization of several major mitochondrial complexes in cells. ATP synthases and respiratory complexes segregate into curved and flat crista membrane domains, respectively. Respiratory complexes I, III, and IV assemble into a respirasome supercomplex, from which we determined a native 5-angstrom (Å) resolution structure showing binding of electron carrier cytochrome . Combined with single-particle cryo-electron microscopy at 2.4-Å resolution, we model how the respiratory complexes organize inside native mitochondria.

PubMed: 40112058
DOI: 10.1126/science.ads8738

Experimental method

ELECTRON MICROSCOPY (2.51 Å)