9EYG
Structure of Tannerella forsythia endopeptidase O (TfPepO)
Summary for 9EYG
| Entry DOI | 10.2210/pdb9eyg/pdb |
| Descriptor | Peptidase family M13, 1,2-ETHANEDIOL, DI(HYDROXYETHYL)ETHER, ... (7 entities in total) |
| Functional Keywords | protease tannerella forsythia, hydrolase |
| Biological source | Tannerella forsythia |
| Total number of polymer chains | 2 |
| Total formula weight | 151875.14 |
| Authors | Zak, K.M.,Grudnik, P.,Waligorska, I.,Ksiazek, M. (deposition date: 2024-04-09, release date: 2025-10-29, Last modification date: 2026-05-13) |
| Primary citation | Waligorska, I.,Zak, K.M.,Mikrut, N.,Budziaszek, J.,Medviediev, V.,Bielecka, E.,Kantyka, T.,Koziel, J.,Thogersen, I.B.,Enghild, J.J.,Grudnik, P.,Potempa, J.,Ksiazek, M. Periodontopathogens degrade angiotensin I from the human renin-angiotensin system through surface-attached proteases. Sci Rep, 15:43309-43309, 2025 Cited by PubMed Abstract: The renin-angiotensin system (RAS) has its effects through biologically-active peptides, the angiotensins (Ang). The angiotensinogen-derived precursor, Ang I, is cleaved either to proinflammatory Ang II, which increases blood pressure or to Ang 1-7, which has opposite effects to Ang II. Here, we show that Porphyromonas gingivalis (Pg) and Tannerella forsythia (Tf), endogenous oral pathogens, direct the RAS to generate Ang 1-7 through the actions of the endopeptidases O PgPepO and TfPepO, respectively. The thermophilic PepOs metalloproteases preferred large hydrophobic amino acids at the carbonyl terminus of scissile peptide bonds (P1' position), and TfPepO, in contrast to all known homologous proteases, hydrolyzed substrates distant to both termini. The crystal structures revealed exceptionally wide entrances to the catalytic cleft, which explains the unique properties of TfPepO. Multiple immunoassays showed that PepOs attached to bacterial cell surfaces are released in outer membrane vesicles. Moreover, PepO was responsible for Ang I hydrolysis by Pg and Tf. Finally, PepO deletion reduced only the virulence of Tf using the Galleria mellonella model. Thus, our data show that PepOs are the only proteases of Pg and Tf, which may modulate RAS through AngI hydrolysis. PubMed: 41310390DOI: 10.1038/s41598-025-27891-0 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.8 Å) |
Structure validation
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