9EYG
Structure of Tannerella forsythia endopeptidase O (TfPepO)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 193 |
| Detector technology | PIXEL |
| Collection date | 2020-06-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 95.414, 95.414, 301.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.750 - 1.800 |
| R-factor | 0.1955 |
| Rwork | 0.194 |
| R-free | 0.22330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.973 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MoRDa |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.800 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.080 | 1.132 |
| Number of reflections | 148384 | 14612 |
| <I/σ(I)> | 16.92 | |
| Completeness [%] | 100.0 | 99.99 |
| Redundancy | 9.3 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.3 M sodium fluoride, 0.3 M sodium bromide, 0.3 M sodium iodide, 0.1M buffer system containing imidazole / MES mix, 40% (v/v) ethylene glycol, 20% (w/v) PEG 8000, pH=6.5 |
