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9ENC

Human pseudouridine synthase 3 (PUS3 R116A mutant) and one tRNA-Gln

Summary for 9ENC
Entry DOI10.2210/pdb9enc/pdb
EMDB information16917 16926 19831
DescriptortRNA pseudouridine(38/39) synthase, tRNA-Gln (2 entities in total)
Functional Keywordsrna modification, pseudouridylation, trna, homodimer, rna binding protein
Biological sourceHomo sapiens (human)
More
Total number of polymer chains3
Total formula weight135325.44
Authors
Lin, T.-Y.,Jezowski, J.,Glatt, S. (deposition date: 2024-03-12, release date: 2024-07-10, Last modification date: 2024-07-24)
Primary citationLin, T.Y.,Kleemann, L.,Jezowski, J.,Dobosz, D.,Rawski, M.,Indyka, P.,Wazny, G.,Mehta, R.,Chramiec-Glabik, A.,Koziej, L.,Ranff, T.,Fufezan, C.,Wawro, M.,Kochan, J.,Bereta, J.,Leidel, S.A.,Glatt, S.
The molecular basis of tRNA selectivity by human pseudouridine synthase 3.
Mol.Cell, 84:2472-2489.e8, 2024
Cited by
PubMed Abstract: Pseudouridine (Ψ), the isomer of uridine, is ubiquitously found in RNA, including tRNA, rRNA, and mRNA. Human pseudouridine synthase 3 (PUS3) catalyzes pseudouridylation of position 38/39 in tRNAs. However, the molecular mechanisms by which it recognizes its RNA targets and achieves site specificity remain elusive. Here, we determine single-particle cryo-EM structures of PUS3 in its apo form and bound to three tRNAs, showing how the symmetric PUS3 homodimer recognizes tRNAs and positions the target uridine next to its active site. Structure-guided and patient-derived mutations validate our structural findings in complementary biochemical assays. Furthermore, we deleted PUS1 and PUS3 in HEK293 cells and mapped transcriptome-wide Ψ sites by Pseudo-seq. Although PUS1-dependent sites were detectable in tRNA and mRNA, we found no evidence that human PUS3 modifies mRNAs. Our work provides the molecular basis for PUS3-mediated tRNA modification in humans and explains how its tRNA modification activity is linked to intellectual disabilities.
PubMed: 38996458
DOI: 10.1016/j.molcel.2024.06.013
PDB entries with the same primary citation
Experimental method
ELECTRON MICROSCOPY (3.36 Å)
Structure validation

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PDB entries from 2024-11-06

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