9EHB
Structure of short Lettuce aptamer (C14T variant) bound with TO1-biotin.
Summary for 9EHB
| Entry DOI | 10.2210/pdb9ehb/pdb |
| Descriptor | DNA 53-mer, POTASSIUM ION, MAGNESIUM ION, ... (6 entities in total) |
| Functional Keywords | structure of short lettuce aptamer (c14t variant) bound with to1-biotin., dna |
| Biological source | synthetic construct |
| Total number of polymer chains | 1 |
| Total formula weight | 17493.65 |
| Authors | Koomullam, N.,Batchelder-Schwab, E.,Mao, C. (deposition date: 2024-11-22, release date: 2026-05-27, Last modification date: 2026-06-03) |
| Primary citation | Kuo, Y.A.,Chen, Y.I.,Siraj, N.,He, Y.,Yang, Z.,Wang, Y.,Batchelder-Schwab, E.J.,Korkmaz, Z.,Yonas, S.,Nguyen, T.D.,Hong, S.,Nguyen, A.T.,Kim, S.,Seifi, S.,Fan, P.H.,Wu, Y.,Liu, H.W.,Lu, Y.,Ren, P.,Mao, C.,Yeh, H.C. Fluorogenic Aptamer Optimization on a Massively Parallel Sequencing Platform. ACS Sens, 11:3587-3600, 2026 Cited by PubMed Abstract: Fluorogenic aptamers (FAPs) are increasingly important tools for cellular sensing and pathogen diagnostics. However, enhancing their performance remains a significant challenge. Here, we introduce a massively parallel approach to optimize the DNA-based FAP Lettuce using repurposed next-generation sequencing flow cells. By replacing Lettuce's cognate fluorogen, DFHBI-1T, with TO1-biotin, we achieve a 4-fold ensemble fluorescence enhancement, and a broader fluorescence lifetime modulation range from 4.7 to 6.0 ns. Through screening 8821 Lettuce variants complexed with TO1-biotin on a chip, we identify the C14T mutant which exhibits an improved dissociation constant, increased quantum yield, extended fluorescence lifetime, and enhanced emission intensity. Co-crystal structures of the aptamer/fluorogen complexes reveal that π-π stacking interactions are critical for the stable coordination of TO1-biotin within Lettuce. When tested in a cellular environment, both the screening-identified C14T mutant and a structure-guided rationally designed variant, C14dU, exhibit 15% and 18% stronger fluorescence intensities, respectively, compared with canonical Lettuce. Our massive screening and molecular dynamics simulation pipeline enable efficient FAP optimization without prior structural knowledge, yielding not only improved probes for fluorescence sensing but also deeper insights into aptamer-fluorogen interactions. PubMed: 42112875DOI: 10.1021/acssensors.5c04046 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.03 Å) |
Structure validation
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